Transfection of the int-1 mammary oncogene in cuboidal RAC mammary cell line results in morphological transformation and tumorigenicity.
AUTOR(ES)
Rijsewijk, F
RESUMO
The int-1 gene is often activated by proviral insertion in mouse mammary tumors. Direct evidence for the normal function of this gene and its role in tumorigenesis has therefore been lacking. To examine possible biological effects of int-1 activation in in vitro cell systems, we have constructed recombinant molecules of genomic int-1 DNA, transcriptionally activated by retroviral promoters. Transfection of these constructs into cuboidal RAC311C mammary cells leads to morphological transformation of the cells and rapid tumorigenicity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=553367Documentos Relacionados
- Construction of a retroviral cDNA version of the int-1 mammary oncogene and its expression in vitro.
- The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences.
- E1A oncogene induction of cytolytic susceptibility eliminates sarcoma cell tumorigenicity.
- Retroviral expression of the human IL-2 gene in a murine T cell line results in cell growth autonomy and tumorigenicity.
- Activation of int-1 and int-2 mammary oncogenes in hormone-dependent and -independent mammary tumors of GR mice.