Transfer of Newly Synthesized Proteins from Schwann Cells to the Squid Giant Axon
AUTOR(ES)
Lasek, R. J.
RESUMO
The squid giant axon is presented as a model for the study of macromolecular interaction between cells in the nervous system. When the isolated giant axon was incubated in sea water containing [3H]leucine for 0.5-5 hr, newly synthesized proteins appeared in the sheath and axoplasm as demonstrated by: (i) radioautography, (ii) separation of the sheath and axoplasm by extrusion, and (iii) perfusion of electrically excitable axons. The absence of ribosomal RNA in the axoplasm [Lasek, R. J. et al. (1973) Nature 244, 162-165] coupled with other evidence indicates that the labeled proteins that are found in the axoplasm originate in the Schwann cells surrounding the axon. Approximately 50% of the newly synthesized Schwann cell proteins are transferred to the giant axon. These transferred proteins are soluble for the most part and range in molecular size from 12,000 to greater than 200,000 daltons. It is suggested that proteins transferred from the Schwann cell to the axon have a regulatory role in neuronal function.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388189Documentos Relacionados
- Transport of cytoskeletal elements in the squid giant axon.
- Excitation of the squid giant axon by general anaesthetics.
- Protein synthesis in the isolated giant axon of the squid.
- Diphenylhydantoin Inhibition of Sodium Conductance in Squid Giant Axon
- Potassium conductance of the squid giant axon is modulated by ATP.
