Transfer of phosphoethanolamine residues from phosphatidylethanolamine to the membrane-derived oligosaccharides of Escherichia coli.
AUTOR(ES)
Miller, K J
RESUMO
The membrane-derived oligosaccharides (MDO) of Escherichia coli are periplasmic constituents composed of glucose residues linked by beta-1,2 and beta-1,6 glycosidic bonds. MDO are substituted with phosphoglycerol, phosphoethanolamine, and succinic acid moieties. The phosphoglycerol residues present on MDO are derived from phosphatidylglycerol (B. J. Jackson and E. P. Kennedy, J. Biol. Chem. 258:2394-2398, 1983), but evidence as to the source of the phosphoethanolamine residues has been lacking. We now report that phosphatidylethanolamine, exogenously added to intact cells of E. coli, provides a source of phosphoethanolamine residues that are transferred to MDO. The biosynthesis of phosphoethanolamine-labeled MDO is osmotically regulated, with maximum synthesis occurring during growth in medium of low osmolarity.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211833Documentos Relacionados
- Osmotic regulation of biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
- Osmotic regulation and the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
- Mapping of a locus (mdoA) that affects the biosynthesis of membrane-derived oligosaccharides in Escherichia coli.
- An essential function for acyl carrier protein in the biosynthesis of membrane-derived oligosaccharides of Escherichia coli.
- Isolation and characterization of Escherichia coli mutants blocked in production of membrane-derived oligosaccharides.
