Transformation of rat fibroblast cells with early mutants of polyoma (tsa) and simian virus 40 (tsA30): occurrence of either A or N transformants depends on the multiplicity of infection.
AUTOR(ES)
Rassoulzadegan, M
RESUMO
Infection of rat fibroblasts with early mutants of polyoma virus (tsa) or simian virus 40 (tsA30) leads to the establishment of either temperature-independent A transformants or N transformants temperature-sensitive for the expression of the transformed phenotype. The choice between the A- and N-transformed states is not only dependent, as we reported previously (Rassoulzadegan et al., j. Virol., 28:421-426, 1978), on the growth conditions after infection, but is also a function of the multiplicity of infection (MOI); high MOI led to the predominant occurrence of A derivatives, and lower MOI led to that of N transformants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=288616Documentos Relacionados
- Phenotype-specific phosphorylation of simian virus 40 tsA mutant large T antigens in tsA N-type and A-type transformants.
- Temperature dependency for maintenance of transformation in mouse cells transformed by simian virus 40 tsA mutants.
- Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: effect of transformation technique on the transformed phenotype.
- Flat revertants of temperature-insensitive transformants induced by simian virus 40 tsA mutants lose their ability to express T-antigen.
- Growth control in simian virus 40-transformed rat cells: temperature-independent expression of the transformed phenotype in tsA transformants derived by agar selection.