Transgene-mediated cosuppression in the C. elegans germ line
AUTOR(ES)
Dernburg, Abby F.
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
Functional silencing of chromosomal loci can be induced by transgenes (cosuppression) or by introduction of double-stranded RNA (RNAi). Here, we demonstrate the generality of and define rules for a transgene-mediated cosuppression phenomenon in the Caenorhabditis elegans germ line. Functional repression is not a consequence of persistent physical association between transgenes and endogenous genes or of mutations in affected genes. The cosuppression mechanism likely involves an RNA mediator that defines its target specificity, reminiscent of RNAi. Cosuppression is strongly abrogated in rde-2 and mut-7 mutants, but is not blocked in an rde-1 mutant, indicating that cosuppression and RNAi have overlapping but distinct genetic requirements.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316736Documentos Relacionados
- An active role for endogenous β-1,3-glucanase genes in transgene-mediated co-suppression in tobacco
- Transgene-mediated post-transcriptional gene silencing is inhibited by 3′ non-coding sequences in Paramecium
- Transgene-mediated and elicitor-induced perturbation of metabolic channeling at the entry point into the phenylpropanoid pathway
- A C. elegans patched gene, ptc-1, functions in germ-line cytokinesis
- Phenotypic and Molecular Analysis of Mes-3, a Maternal-Effect Gene Required for Proliferation and Viability of the Germ Line in C. Elegans