Translation of Rous sarcoma virus RNA in a cell-free system from ascites Krebs II cells.
AUTOR(ES)
von der Helm, K
RESUMO
The template activities of the 60-70S RNA complex and of the 30-40S subunit RNA species of Rous sarcoma virus were tested in a cell-free protein-synthesizing system from mouse ascites Krebs II cells. Stimulation of protein synthesis over the endogenous background was about 2-fold with 30-40S viral RNA and about 1.3-fold with 60-70S viral RNA as template. Analysis by sodium dodecyl sulfate-gel electrophoresis showed that the predominant polypeptide synthesized in vitro in response to 30-40S RNA of Rous sarcoma virus had a molecular weight of 75,000-80,000. This polypeptide could be precipitated by antiserum against the group-specific antigens of the virus, although its molecular weight is higher than that of virion group-specific antigen proteins. Analysis of tryptic digests of the protein made in vitro indicates similarity to tryptic digests from authentic virion group-specific proteins. It is concluded that part of the RNA from Rous sarcoma virus is translated in vitro into a high-molecular-weight protein, perhaps a precursor of the virion group-specific proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=432364Documentos Relacionados
- Translation of murine leukemia virus RNA in cell-free systems from animal cells.
- Cell-free translation of virion RNA from nondefective and transformation-defective Rous sarcoma viruses.
- Cell-Free Translation of Virion RNA from Nondefective and Transformation-Defective Rous Sarcoma Viruses
- Synthesis of α and β Chains of Rabbit Hemoglobin in a Cell-Free Extract from Krebs II Ascites Cells
- env Gene of Rous sarcoma virus: identification of the gene product by cell-free translation.
