Translational Repression in the Arginine System of Escherichia coli*
AUTOR(ES)
McLellan, William L.
RESUMO
Translation of bacterial mRNA, divorced from transcription, has been obtained for enzymes of arginine synthesis; evidence has been acquired for repression by arginine at the level of translation. mRNAs for acetylornithinase and ornithine transcarbamylase were accumulated by arginine starvation of argR+ and argR- arginine auxotrophs derived from Escherichia coli K12. Further transcription was inhibited with rifampicin or miracil D, and enzyme formation was measured in the presence of either an excess of, or a restricted supply of, arginine. For the argR+ strain 961, little mRNA was found without starvation; for the argR- strain 977, a considerable amount of mRNA was demonstrated even without starvation. There was relatively little translation for the argR+ strain, but not for the argR- strain, in the presence of excess arginine, apparently due to an accelerated degradation of mRNA in the argR+ strain under repressive conditions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=283415Documentos Relacionados
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