Transposition and transposition immunity of transposon Tn3 derivatives having different ends.

AUTOR(ES)

Arthur, A

RESUMO

Novel Tn1/3 derivatives that contained either two left- or two right-hand ends of the transposon were constructed in a small plasmid. Both transposed at reasonable frequencies to give normal transposition products, suggesting that only the 38-bp inverted repeats of Tn3 are essential for transposition. Plasmids containing transposon derivatives with only one end (either left or right) undergo transposase-dependent transposition between replicons at much lower frequencies, resulting in co-integrate molecules in which there is no substantial duplication of transposon DNA and that appear to be simple fusions of the two plasmids. Both the right and left halves of the transposon are separately able to confer transposition immunity to the plasmid, this immunity being inseparably linked to transposition proficiency and specificity.

Documentos Relacionados

• Identification of a transposon Tn3 sequence required for transposition immunity.
• Nucleotide sequences required for Tn3 transposition immunity.
• Tn 10 transposition in vivo: temporal separation of cleavages at the two transposon ends and roles of terminal basepairs subsequent to interaction of ends.
• Mutational analysis of insertion sequence 50 (IS50) and transposon 5 (Tn5) ends.
• Isolation and analysis of inhibitors of transposon Tn3 site-specific recombination.