Treatment of Human Immunodeficiency Virus Type 1 Virions Depleted of Cyclophilin A by Natural Endogenous Reverse Transcription Restores Infectivity

AUTOR(ES)

Khan, Mahfuz

FONTE

American Society for Microbiology

RESUMO

We have previously shown that virions with nef deleted can be restored to wild-type infectivity by treatment to induce natural endogenous reverse transcription (NERT). Since Nef and cyclophilin A (CyPA) appear to act in similar ways on postentry events, we determined whether NERT treatment would restore infectivity to virions depleted of CyPA. Our results show that the infectivity of virions depleted of CyPA by treatment with cyclosporine A could be restored by NERT treatment, while mutants in the CyPA binding loop of capsid could only be partially restored. These results suggest that CyPA is involved in some aspect of the uncoating process.

Documentos Relacionados

• Target Cell Cyclophilin A Modulates Human Immunodeficiency Virus Type 1 Infectivity
• Restoration of Wild-Type Infectivity to Human Immunodeficiency Virus Type 1 Strains Lacking nef by Intravirion Reverse Transcription
• The importance of the A-rich loop in human immunodeficiency virus type 1 reverse transcription and infectivity.
• Reversion of a Human Immunodeficiency Virus Type 1 Matrix Mutation Affecting Gag Membrane Binding, Endogenous Reverse Transcriptase Activity, and Virus Infectivity
• Active-site residues of cyclophilin A are crucial for its incorporation into human immunodeficiency virus type 1 virions.