Trehalose Phosphate Synthesis in Streptomyces hygroscopicus: Purification of Guanosine Diphosphate d-Glucose: d-Glucose-6-Phosphate 1-Glucosyl-Transferase
AUTOR(ES)
Elbein, Alan D.
RESUMO
Guanosine diphosphate d-glucose:d-glucose-6-phosphate 1-glucosyl-transferase was purified approximately 100-fold from extracts of Streptomyces hygroscopicus. The purified enzyme catalyzed the transfer of glucose from guanosine diphosphate-d-glucose to glucose-6-phosphate to form trehalose phosphate and guanosine diphosphate. The enzyme was specific for these two substrates and was stimulated by the addition of magnesium ions. The product was characterized as α-α-trehalose-6-phosphate by its physical and chemical properties. The enzyme was present in a large number of Streptomyces species, suggesting that this group of organisms synthesized trehalose phosphate in a unique manner. This enzyme was not detected in fungi, since these organisms utilized uridine diphosphate-d-glucose as the glucosyl donor.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=315220Documentos Relacionados
- d-Glucose 6-Phosphate Cycloaldolase: Inhibition Studies and Aldolase Function 1
- Uridine Diphosphate d-Glucose Dehydrogenase of Aerobacter aerogenes
- Isolation of thymidine diphosphate D-glucose, thymidine diphosphate D-galactose, and thymidine diphosphate 4-acetamido-4, 6-dideoxy-D-galactose from Pasteurella pseudotuberculosis.
- GLYCOGEN SYNTHESIS FROM GLUCOSE, GLUCOSE-6-PHOSPHATE, AND URIDINE DIPHOSPHATE GLUCOSE IN MUSCLE PREPARATIONS*
- Trehalose Metabolism in Germinating Spores of Streptomyces hygroscopicus