Triiodothyronine Radioimmunoassay

AUTOR(ES)

Lieblich, Jeffrey

RESUMO

Highly specific antisera to triiodothyronine (T3) were prepared by immunization of rabbits with T3-bovine serum albumin conjugates. Antisera with T3 binding capacity of up to 600 ng/ml were obtained. The ability of various thyronine derivatives to inhibit the binding of T3-125I to anti-T3 serum was found to vary considerably. l-T3, d-T3 and several triiodoanalogues were potent inhibitors of the reaction. Little inhibition of T3-125I binding was produced by l-thyroxine (T4) or other tetraiodo- analogues, thyronine or iodotyrosines. Chromatography of several T4 preparations indicated that most of their very slight activity could be ascribed to contamination with T3.

Documentos Relacionados

• The radioimmunoassay of triiodothyronine and its clinical application.
• Direct Radioimmunoassay of Nuclear 3,5,3′ Triiodothyronine in Rat Anterior Pituitary
• A Radioimmunoassay for Measurement of 3,3′, 5′-Triiodothyronine (Reverse T3)
• Serum triiodothyronine: measurements in human serum by radioimmunoassay with corroboration by gas-liquid chromatography
• A New Radioimmunoassay for Plasma l-Triiodothyronine: Measurements in Thyroid Disease and in Patients Maintained on Hormonal Replacement