Triple Decoding of Hepatitis C Virus RNA by Programmed Translational Frameshifting
AUTOR(ES)
Choi, Jinah
FONTE
American Society for Microbiology
RESUMO
Ribosomes can be programmed to shift from one reading frame to another during translation. Hepatitis C virus (HCV) uses such a mechanism to produce F protein from the −2/+1 reading frame. We now report that the HCV frameshift signal can mediate the synthesis of the core protein of the zero frame, the F protein of the −2/+1 frame, and a 1.5-kDa protein of the −1/+2 frame. This triple decoding function does not require sequences flanking the frameshift signal and is apparently independent of membranes and the synthesis of the HCV polyprotein. Two consensus −1 frameshift sequences in the HCV type 1 frameshift signal facilitate ribosomal frameshifts into both overlapping reading frames. A sequence which is located immediately downstream of the frameshift signal and has the potential to form a double stem-loop structure can significantly enhance translational frameshifting in the presence of the peptidyl-transferase inhibitor puromycin. Based on these results, a model is proposed to explain the triple decoding activities of the HCV ribosomal frameshift signal.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=151691Documentos Relacionados
- Programmed translational frameshifting.
- Kinetics of Ribosomal Pausing during Programmed −1 Translational Frameshifting
- Identification of programmed translational -1 frameshifting sites in the genome of Saccharomyces cerevisiae
- Programmed translational –1 frameshifting on hexanucleotide motifs and the wobble properties of tRNAs
- Nonlinearity in genetic decoding: Homologous DNA replicase genes use alternatives of transcriptional slippage or translational frameshifting