Two-dimensional electrophoresis of plasma membranes, showing differences among wild-type and abnormal ascospore mutant strains of Neurospora crassa.
AUTOR(ES)
Nasrallah, J B
RESUMO
Plasma membranes isolated from vegetative cultures of wild-type Neurospora crassa were analyzed by two-dimensional electrophoresis, followed by staining with silver nitrate to visualize proteins and fluorescein-labeled concanavalin A to visualize glycosylated subunits. Mycelial plasma membranes from strains carrying mutations affecting ascospores were also analyzed. Two of the mutant strains were shown to have aberrant two-dimensional membrane subunit patterns. The correlation of these abnormalities with the known electron microscopic evidence for aberrations of their ascospore-delimiting membrane during ascospore genesis is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=217839Documentos Relacionados
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