Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.
AUTOR(ES)
Martinussen, J
RESUMO
Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene--the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=176586Documentos Relacionados
- Two developmental genes encoding sigma factor homologs are arranged in tandem in Bacillus subtilis.
- Independent genes for two threonyl-tRNA synthetases in Bacillus subtilis.
- Evidence for two structural genes for alkaline phosphatase in Bacillus subtilis.
- Two thymidylate synthetases in Bacillus subtilis.
- Cloning and characterization of a cluster of genes encoding polypeptides present in the insoluble fraction of the spore coat of Bacillus subtilis.