Two levels of regulation of beta-interferon gene expression in human cells.
AUTOR(ES)
Raj, N B
RESUMO
We cloned alpha- and beta-interferon cDNA and used them as specific probes to determine the relative levels of interferon mRNA in human fibroblasts cells induced with poly(rI).poly(rC) or Newcastle disease virus to synthesize interferon. Both inducers activated only the beta-interferon gene; however, the half life of beta-interferon mRNA in cells induced with virus was substantially longer than in poly(rI).poly(rC)-induced cells. The transcription rate of beta-interferon RNA sequences was examined in nuclei isolated from poly(rI).poly(rC)-induced cells; it was found that the induction leads to transcriptional activation of the beta-interferon gene and that the shutoff period when no interferon synthesis or cytoplasmic betamRNA are detected. Thus, the synthesis of beta interferon in poly(rI).poly-(rC)-induced human fibroblasts is controlled both by activation of transcription of the beta-interferon gene and by alteration of the beta-interferon mRNA stability.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=394171Documentos Relacionados
- Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.
- Postinduction turnoff of beta-interferon gene expression.
- Control of beta-interferon expression in murine embryonal carcinoma F9 cells.
- Two different virus-inducible elements are required for human beta-interferon gene regulation.
- NF-kappa B-independent activation of beta-interferon expression in mouse F9 embryonal carcinoma cells.