Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803

AUTOR(ES)

Barkley, Sam J.

FONTE

American Society for Microbiology

RESUMO

Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His6-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; KmIPP was 52 μM, and kcatIPP was 0.23 s−1.

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