Ultrastructural localization of alkaline phosphatase in the blue-green bacterium Plectonema boryanum.
AUTOR(ES)
Doonan, B B
RESUMO
Histochemical techniques applied at the ultrastructural level have clearly established the periplasmic space as the site of alkaline phosphatase activity in Plectonema boryanum. Considerable enzyme activity is found after the organism is placed in a phosphate-free medium for 5 days. This activity is found only in the cellular fraction of the culture with no activity present in the culture medium. Localization of the site of enzyme activity in cells was investigated by a modification of the method of Costerton. Unfixed cells were reacted with calcium nitrate, which acts as the initial capture reagent. After this deposition, the cells were suspended in 2% lead nitrate to convert the calcium phosphate to more electron-dense lead phosphate. The majority of activity appears associated with layer 3 (periplasmic space) of the cell wall.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235599Documentos Relacionados
- Ethionine-resistant mutants of the filamentous blue-green alga Plectonema boryanum.
- Endogenous Dark Respiration of the Blue-Green Alga, Plectonema boryanum
- LPP-1 Infection of the Blue-Green Alga Plectonema boryanum: I. Electron Microscopy
- LPP-1 Infection of the Blue-Green Alga Plectonema boryanum: III. Protein Synthesis
- LPP-1 Infection of the Blue-Green Alga Plectonema boryanum: II. Viral Deoxyribonucleic Acid Synthesis and Host Deoxyribonucleic Acid Breakdown