Ultrastructural localization of endogenous peroxidase in human parotid and submandibular glands.

AUTOR(ES)

Riva, A

RESUMO

The localization of endogenous peroxidase was studied in human parotid and submandibular glands using the medium of Strum & Karnovsky either at pH 7 or at pH 8.3, after a short fixation of the tissues with a low concentration of glutaraldehyde. At pH 7 reaction product was found in seromucous cells of both glands in the cisternae of the rough endoplasmic reticulum, in the elements of the Golgi apparatus, and in secretory products. However, while in the parotid gland all secretory granules showed peroxidase activity, in the submandibular gland some of the granules were left unstained. At pH 8.3 the pattern of reactivity was practically unchanged in the submandibular gland, whereas the amount of precipitate noticeable in the secretory granules of the parotid gland was greatly reduced. The method of Novikoff et al. for catalase revealed the presence of a number of small bodies having in the characteristics of peroxisomes. Acid phosphatase was not demonstrable in the secretory granules of either parotid or submandibular glands. The lead precipitate indicating enzyme activity was observed only in lysosomes and, occasionally, in the elements of the Golgi apparatus of some sermucous cells.

Documentos Relacionados

• Ultrastructural localisation of lactoferrin and glycoprotein in human bronchial glands.
• Ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands.
• Human herpes virus 6 and endogenous biotin in salivary glands.
• Nerve terminals and cellular junctions in young and adult mouse submandibular glands.
• Identification and characterization of a Candida albicans-binding proteoglycan secreted from rat submandibular salivary glands.