Unintegrated viral DNA is synthesized in the cytoplasm of avian sarcoma virus-transformed duck cells by viral DNA polymerase.
AUTOR(ES)
Varmus, H E
RESUMO
We have examined the location, structure, and mechanism of synthesis of unintegrated viral DNA present in fully transformed cultures of avian sarcoma virus-infected duck cells. De novo synthesis of the unintegrated forms several weeks after the initial infection was documented by labeling unintegrated DNA in both strands with 5-bromodeoxyuridine. The unintegrated DNA is synthesized in, and probably confined to, the cytoplasm, and it consists of duplexes of short "plus" strands (ca. 0.5 X 10(6) to 1.0 X 10(6) daltons) and "minus" strands the length of a subunit of the viral genome (ca. 2.5 X 10(6) to 3.0 X 10(6) daltons). The structure of the duplex and the mode of incorporation of density label support the hypothesis that the unintegrated DNA is synthesized from an RNA templated by virus-coded DNA polymerase.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=515583Documentos Relacionados
- Infectivity of Proviral DNA from Avian Sarcoma Virus-Transformed Mammalian Cells
- DNA methylation and transcriptional controls of proviral DNA in avian sarcoma virus-transformed mammalian cells.
- Membrane Proteins of Uninfected and Rous Sarcoma Virus-Transformed Avian Cells
- Characterization of Morphologic Revertants of Murine and Avian Sarcoma Virus-Transformed Cells
- Peptide analysis of the transformation-specific antigen from avian sarcoma virus-transformed cells.