Use of a Single, Triplicate Arbitrarily Primed-PCR Procedure for Molecular Fingerprinting of Lactic Acid Bacteria†
AUTOR(ES)
Cusick, Sean M.
FONTE
American Society for Microbiology
RESUMO
Arbitrarily primed (AP)-PCR can be used to generate characteristic DNA fingerprint patterns. However, small changes in reaction conditions can cause band irreproducibility. In this study, a single methodology encompassing triplicate reactions, which were intentionally exposed to three different annealing temperatures, enabled bands that were reproducibly generated to be recognized. A single triplicate AP-PCR (TAP-PCR) procedure, using an 18-mer primer, was developed and used to fingerprint representative isolates from the major genera of lactic acid bacteria and Bifidobacterium to the strain level.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=101479Documentos Relacionados
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