Use of Gas Chromatography for Determining Catabolic Products of Arginine by Bacteria

AUTOR(ES)

Moss, C. Wayne

RESUMO

A rapid and sensitive procedure for determining catabolic products of arginine metabolism by bacteria was developed. The method consists of inoculating a solution of L-arginine with a heavy cell suspension of the test organism. After a 2-hr incubation period, dissimilation products (citrulline, ornithine, agmatine, putrescine) are converted to volatile derivatives and analyzed by gas-liquid chromatography. Compared with conventional microbiological tests, the new procedure is rapid and can be used for sensitive quantitative measurements of specific metabolites from arginine.

Documentos Relacionados

• Use of Gas-Liquid Chromatography to Determine the End Products of Growth of Lactic Acid Bacteria
• Use of Gas Chromatography for Detecting Ornithine and Lysine Decarboxylase Activity in Bacteria
• Detection of Arginine Dihydrolase in Nonfermentative Gram-Negative Bacteria by Use of Thin-Layer Chromatography
• Rapid and Sensitive Detection of Bacteria by Gas Chromatography
• Detection and Identification of Bacteria by Gas Chromatography1