Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.
AUTOR(ES)
Flinn, H
RESUMO
Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1 site-specific recombination.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=209887Documentos Relacionados
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