Use of histone antibodies for studying chromatin topography and the phosphorylation of chromatin subunits.
AUTOR(ES)
Muller, S
RESUMO
Polyclonal and monoclonal antibodies specific for histones as well as sera directed against synthetic peptides of histones were used to probe the topography of chromatin subunits. In native chromatin, the regions corresponding to residues 130-135 of H3 and 6-18 of H2B were found to be exposed and able to interact with antibodies whereas the regions 26-35 and 36-43 of H2B and 80-89 and 85-102 of H4 were not. In vitro phosphorylation of H3 and H5 in native chromatin or of H3 in H1/H5-depleted chromatin led to a marked drop in the binding of antibodies specific for residues 130-135 of H3 and 6-18 of H2B. Phosphorylation of H1/H5-depleted chromatin also altered the degree of exposure of certain H2A epitopes but it did not affect the surface accessibility of residues 1-11 of H2B.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=557704Documentos Relacionados
- The distribution of histone H1 subfractions in chromatin subunits.
- Immunochemical localization of the C-terminal hexapeptide of histone H3 at the surface of chromatin subunits.
- Studies on chromatin. II. Isolation and characterization of chromatin subunits.
- Identification of nonhistone chromatin proteins in chromatin subunits.
- Structure of transcriptionally-active chromatin subunits.
