Use of Protein in Extraction and Stabilization of Nitrate Reductase 1
AUTOR(ES)
Schrader, L. E.
RESUMO
The in vitro instability of nitrate reductase (EC 1.6.6.1) activity from leaves of several species of higher plants was investigated. Decay of activity was exponential with time, suggesting that an enzyme-catalyzed reaction was involved. The rate of decay of nitrate reductase activity increased as leaf age increased in all species studied. Activity was relatively stable in certain genotypes of Zea mays L., but extremely unstable in others. In all genotypes of Avena sativa L. and Nicotiana tabacum L. studied, nitrate reductase was unstable. Addition of 3% (w/v) bovine serum albumin or casein to extraction media prevented or retarded the decay of nitrate reductase activity for several hours. In addition, the presence of bovine serum albumin or casein in the enzyme homogenate markedly increased nitrate reductase activity (up to 15-fold), especially in older leaf tissue.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=541425Documentos Relacionados
- Nitrate Reductase Activity and Protein Concentration of Two Populas Clones 1
- Nitrate Uptake and Induction of Nitrate Reductase in Excised Corn Roots 1
- Overexpression of Nitrate Reductase in Tobacco Delays Drought-Induced Decreases in Nitrate Reductase Activity and mRNA1
- Characteristics of a Nitrate Reductase in a Barley Mutant Deficient in NADH Nitrate Reductase 1
- Comparative Induction of Nitrate Reductase by Nitrate and Nitrite in Barley Leaves 1