Use of Quantitative PCR To Measure Density of Borrelia burgdorferi in the Midgut and Salivary Glands of Feeding Tick Vectors

AUTOR(ES)

Piesman, Joseph

FONTE

American Society for Microbiology

RESUMO

Quantitative real-time PCR was used to assay spirochetes in feeding ticks. Spirochetes in tick midguts increased sixfold, from 998 per tick before attachment to 5,884 at 48 h of attachment. Spirochetes in tick salivary glands increased >17-fold, from 1.2 per salivary gland pair before feeding to 20.8 at 72 h postattachment. The period of the most rapid increase in the number of spirochetes in the salivary glands occurred from 48 to 60 h postattachment; this time period coincides with the maximal increase in transmission risk during nymphal tick feeding.

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