Use of recombinant retroviruses to characterize the activity of antiretroviral compounds.
AUTOR(ES)
Stair, R K
RESUMO
This report describes the use of a recombinant murine retrovirus encoding beta-galactosidase (PLJ beta-gal retrovirus) to study the antiretroviral activity of zidovudine (AZT) and other nucleoside analogs. The PLJ beta-gal virus permits the rapid and unequivocal identification of individual virus-infected cells arising from a single cycle of viral replication. With this model system, AZT is shown to completely and irreversibly prevent retrovirus infection of proliferating cell lines as measured by a lack of reporter gene expression. On the other hand, AZT is less effective in protecting growth-arrested cells from retroviral infection. Recombinant retroviruses such as the PLJ beta-gal virus are potentially useful reagents for the identification and characterization of antiretroviral compounds.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=250351Documentos Relacionados
- Quantitative molecular monitoring of human immunodeficiency virus type 1 activity during therapy with specific antiretroviral compounds.
- Structure-activity relationships of new antiviral compounds.
- Use of recombinant retroviruses to study the regulation of integrated adenovirus early promoters.
- Pigmentation after long-term topical use of adrenaline compounds.
- Plasmid-determined resistance to tellurium compounds.