USF-related transcription factor, HIV-TF1, stimulates transcription of human immunodeficiency virus-1.
AUTOR(ES)
Maekawa, T
RESUMO
The transcription factor HIV-TF1, which binds to a region about 60 bp upstream from the enhancer of the human immunodeficiency virus-1 (HIV-1), was purified from human B cells. HIV-TF1 had a molecular weight of 39,000. Binding of HIV-TF1 to the HIV long terminal repeat (LTR) activated transcription from the HIV promoter in vitro. The HIV-TF1-binding site in HIV LTR was similar to the site recognized by upstream stimulatory factor (USF) in the adenovirus major late promoter. DNA-binding properties of HIV-TF1 suggested that HIV-TF1 might be identical or related to USF. Interestingly, treatment of purified HIV-TF1 by phosphatase greatly reduced its DNA-binding activity, suggesting that phosphorylation of HIV-TF1 was essential for DNA binding. The disruption of HIV-TF1-binding site induced a 60% decrease in the level of transcription from the HIV promoter in vivo. These results suggest that HIV-TF1 is involved in transcriptional regulation of HIV-1.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=328710Documentos Relacionados
- Human transcription factor USF stimulates transcription through the initiator elements of the HIV-1 and the Ad-ML promoters.
- Molecular and functional interactions of transcription factor USF with the long terminal repeat of human immunodeficiency virus type 1.
- Human T cell transcription factor GATA-3 stimulates HIV-1 expression.
- Modulation of interleukin-1 beta RNA in monocytic cells infected with human immunodeficiency virus-1.
- A novel Ets-related transcription factor, Elf-1, binds to human immunodeficiency virus type 2 regulatory elements that are required for inducible trans activation in T cells.