UV mutagenesis in Salmonella typhimurium is umuDC dependent despite the presence of samAB.
AUTOR(ES)
Koch, W H
RESUMO
We investigated the role of the umuDC and samAB operons in the UV mutability of Salmonella typhimurium. umuDC is located on the chromosome, whereas samAB resides on the virulence plasmid pSLT. Using allele replacement and plasmid curing techniques, we found that UV mutability was eliminated when any of three different umuDC alleles (umuD1, umuC1, or umuD1 umuC1) were on the chromosome even when samAB was present. We conclude that samAB normally does not complement umuDC function in S. typhimurium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=205931Documentos Relacionados
- Roles of Salmonella typhimurium umuDC and samAB in UV mutagenesis and UV sensitivity.
- Salmonella typhimurium has two homologous but different umuDC operons: cloning of a new umuDC-like operon (samAB) present in a 60-megadalton cryptic plasmid of S. typhimurium.
- Identification of a umuDC locus in Salmonella typhimurium LT2.
- Sequence analysis and mapping of the Salmonella typhimurium LT2 umuDC operon.
- umuDC and mucAB operons whose products are required for UV light- and chemical-induced mutagenesis: UmuD, MucA, and LexA proteins share homology.
