V3 Serological Subtyping of Human Immunodeficiency Virus Type 2 Infection Is Not Relevant
AUTOR(ES)
Plantier, Jean-Christophe
FONTE
American Society for Microbiology
RESUMO
V3 enzyme immunoassays have been shown to discriminate effectively between human immunodeficiency virus type 1 (HIV-1) subtypes. The aim of this study was to investigate the feasibility of V3 serotyping for HIV-2 infection. We serotyped 29 sera with three peptides, corresponding to the V3 loop of subtypes A, B, and D of HIV-2. Sera were collected from HIV-2-infected patients, whose infecting strains were sequenced and subjected to phylogenetic analysis. Our results indicate that HIV-2 serotyping using V3 peptides is not relevant. V3 serotyping data were not consistent with genotyping results. The V3-A and V3-D peptides displayed poor discrimination, and the V3-B peptide was not representative of circulating viruses. Comparison of amino acid sequences and serotype reactivities demonstrated the importance of positions 309 and 314, located on either side of the tip of the V3 loop, in antibody binding.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=88441Documentos Relacionados
- In vivo genetic variability of the human immunodeficiency virus type 2 V3 region.
- Patterns of Changes in Human Immunodeficiency Virus Type 1 V3 Sequence Populations Late in Infection
- Multibranched V3 peptides inhibit human immunodeficiency virus infection in human lymphocytes and macrophages.
- V3 loop of the human immunodeficiency virus type 1 Env protein: interpreting sequence variability.
- Simple determination of human immunodeficiency virus type 1 syncytium-inducing V3 genotype by PCR.
