Vaccinia Virus WR Gene A5L Is Required for Morphogenesis of Mature Virions
AUTOR(ES)
Williams, Ollie
FONTE
American Society for Microbiology
RESUMO
The vaccinia virus WR A5L open reading frame (corresponding to open reading frame A4L in vaccinia virus Copenhagen) encodes an immunodominant late protein found in the core of the vaccinia virion. To investigate the role of this protein in vaccinia virus replication, we have constructed a recombinant virus, vA5Li, in which the endogenous gene has been deleted and an inducible copy of the A5 gene dependent on isopropyl-β-d-thiogalactopyranoside (IPTG) for expression has been inserted into the genome. In the absence of inducer, the yield of infectious virus was dramatically reduced. However, DNA synthesis and processing, viral protein expression (except for A5), and early stages in virion formation were indistinguishable from the analogous steps in a normal infection. Electron microscopy revealed that the major vaccinia virus structural form present in cells infected with vA5Li in the absence of inducer was immature virions. Viral particles were purified from vA5Li-infected cells in the presence and absence of inducer. Both particles contained viral DNA and the full complement of viral proteins, except for A5, which was missing from particles prepared in the absence of inducer. The particles prepared in the presence of IPTG were more infectious than those prepared in its absence. The A5 protein appears to be required for the immature virion to form the brick-shaped intracellular mature virion.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=112500Documentos Relacionados
- Vaccinia Virus Morphogenesis: A13 Phosphoprotein Is Required for Assembly of Mature Virions
- A conditional-lethal vaccinia virus mutant demonstrates that the I7L gene product is required for virion morphogenesis
- The vaccinia virus I1 protein is essential for the assembly of mature virions.
- De novo synthesis of the early transcription factor 70-kilodalton subunit is required for morphogenesis of vaccinia virions.
- A Glutaredoxin, Encoded by the G4L Gene of Vaccinia Virus, Is Essential for Virion Morphogenesis