Vacuolar uptake of host components, and a role for cholesterol and sphingomyelin in malarial infection
AUTOR(ES)
Lauer, Sabine
FONTE
Oxford University Press
RESUMO
Erythrocytes, which are incapable of endocytosis or phagocytosis, can be infected by the malaria parasite Plasmodium falciparum. We find that a transmembrane protein (Duffy), glycosylphosphatidylinositol (GPI)-anchored and cytoplasmic proteins, associated with detergent-resistant membranes (DRMs) that are characteristic of microdomains in host cell membranes, are internalized by vacuolar parasites, while the major integral membrane and cytoskeletal proteins are not. The internalized host proteins and a plasmodial transmembrane resident parasitophorous vacuolar membrane (PVM) protein are detected in DRMs associated with vacuolar parasites. This is the first report of a host transmembrane protein being recruited into an apicomplexan vacuole and of the presence of vacuolar DRMs; it establishes that integral association does not preclude protein internalization into the P.falciparum vacuole. Rather, as shown for Duffy, intracellular accumulation occurs at the same rate as that seen for a DRM-associated GPI-anchored protein. Furthermore, novel mechanisms regulated by the DRM lipids, sphingomyelin and cholesterol, mediate (i) the uptake of host DRM proteins and (ii) maintenance of the intracellular vacuole in the non-endocytic red cell, which may have implications for intracellular parasitism and pathogenesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=313993Documentos Relacionados
- Hepatic uptake and degradation of unilamellar sphingomyelin/cholesterol liposomes: a kinetic study.
- Compositional domain structure in phosphatidylcholine--cholesterol and sphingomyelin--cholesterol bilayers.
- Host Cell P-glycoprotein Is Essential for Cholesterol Uptake and Replication of Toxoplasma gondii*
- Essential role of membrane-attack protein in malarial transmission to mosquito host
- Role for Influenza Virus Envelope Cholesterol in Virus Entry and Infection