Very short patch mismatch repair activity associated with gene dcm is not conferred by a plasmid coding for EcoRII methylase.

AUTOR(ES)

Lieb, M

RESUMO

The only cytosine methylase in Escherichia coli K-12 methylates the second cytosine in the sequence CC (A/T)GG and is encoded by gene dcm. Methylation and very short patch mismatch repair activities lacking in a dcm mutant of E. coli were restored by a plasmid containing the cloned dcm gene. In contrast, plasmids with the gene for EcoRII methylase, which is a homolog of dcm, restored only cytosine methylase activity and not mismatch repair.

Documentos Relacionados

• Binding of the EcoRII methylase to azacytosine-containing DNA.
• A gene required for very short patch repair in Escherichia coli is adjacent to the DNA cytosine methylase gene.
• Autogenous regulation of the EcoRII methylase gene at the transcriptional level: effect of 5-azacytidine.
• Spontaneous Mutation at a 5-Methylcytosine Hotspot Is Prevented by Very Short Patch (Vsp) Mismatch Repair
• The core element of the EcoRII methylase as defined by protease digestion and deletion analysis.