Viral DNA·RNA Hybrids in Cells Infected with Simian Virus: The Simian Virus 40 Transcriptional Intermediates
AUTOR(ES)
Girard, Marc
RESUMO
Brief labeling of cells infected with simian virus 40 with tritiated uridine resulted in the incorporation of part of the label into material that was soluble in 1 M NaCl and sensitive to KOH, with a buoyant density close to that of DNA. The properties of this material suggest that it represents single-stranded nascent molecules of messenger RNA of simian virus 40 hydrogen-bonded over a small portion of their length to viral DNA templates. The name “simian virus 40 transcriptional intermediate molecules” is suggested for these naturally occurring DNA·RNA hybrid molecules. The DNA in the hybrid seems to be in the form of replicative intermediate molecules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388207Documentos Relacionados
- 125I-Labeled DNA·RNA Hybrids in Cytological Preparations
- Fate of viral DNA in nonpermissive cells infected with simian virus 40.
- Loss of Simian Virus 40 DNA-RNA Hybrids from Nitrocellulose Membranes; Implications for the Study of Virus-Host DNA Interactions
- Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
- Viral DNA Synthesis in Cells Infected by Temperature-Sensitive Mutants of Simian Virus 40
