Virus-mediated or transgenic suppression of a G-protein alpha subunit and attenuation of fungal virulence.
AUTOR(ES)
Choi, G H
RESUMO
Strains of the chestnut blight fungus Cryphonectria parasitica harboring RNA viruses of the genus Hypovirus exhibit significantly reduced levels of virulence (called hypovirulence). The accumulation of a heterotrimeric GTP-binding protein (G protein) alpha subunit of the Gi class was found to be reduced in hypovirus-containing C. parasitica strains. Transgenic cosuppression, a phenomenon frequently observed in transgenic plants, reduced the accumulation of this alpha subunit in virus-free fungal strains. Significantly, the resulting transgenic fungal strains were also hypovirulent. These results indicate a crucial role for G-protein-linked signal transduction in fungal pathogenesis and suggest a molecular basis for virus-mediated attenuation of fungal virulence.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=42867Documentos Relacionados
- Biological control of chestnut blight: an example of virus-mediated attenuation of fungal pathogenesis.
- G-protein alpha-subunit expression, myristoylation, and membrane association in COS cells.
- Structural Determinants of G-protein α Subunit Selectivity by Regulator of G-protein Signaling 2 (RGS2)*
- Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery
- Cloning of a Cryptococcus neoformans gene, GPA1, encoding a G-protein alpha-subunit homolog.