Viruses of Entamoeba histolytica III. Properties of the Polyhedral Virus of the HB-301 Strain
AUTOR(ES)
Hruska, Jerome F.
RESUMO
Some biological characteristics and bioassay of a polyhedral virus isolated from normal-appearing strain HB-301 of Entamoeba histolytica are described. The virus produced lysis of susceptible strains of E. histolytica, yet it caused no cytopathological effect in the host strain, HB-301. The virus and host amoeba existed in an equilibrium; approximately 104 mean infective dose units of virus were produced per million HB-301 amoebae. Superinfection and antiviral antiserum treatment failed to disturb this equilibrium permanently. The mechanism of viral persistence in strain HB-301 amoebae remains to be determined. Purification of the virus was attempted. Ninety-nine percent of the viral infectivity was associated with a low-speed pellet which consisted of complexes of virus and cellular membranes. Various treatments of this low-speed pellet failed to release virus. Biologically active, membrane-free virus of low titer was prepared by differential centrifugation of supernatant solutions and employed in electron microscopy and other studies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355068Documentos Relacionados
- Viruses of Entamoeba histolytica V. Ultrastructure of the Polyhedral Virus V301
- Viruses of Entamoeba histolytica II. Morphogenesis of the Polyhedral Particle (ABRM2→HK-9)→HB-301 and the Filamentous Agent (ABRM)2→HK-9
- Viruses of Entamoeba histolytica IV. Studies on the Nucleic Acids of the Filamentous and Polyhedral Viruses
- Viruses of Entamoeba histolytica. VII. Novel beaded virus.
- Viruses of Entamoeba histolytica I. Identification of Transmissible Virus-Like Agents
