Voltage-gated and agonist-mediated rises in intracellular Ca2+ in rat clonal pituitary cells (GH3) held under voltage clamp.
AUTOR(ES)
Benham, C D
RESUMO
1. Intracellular free calcium (Ca2+i) was estimated in single GH3 cells by dual wavelength emission spectrofluorimetry using the Ca2+ indicator dye Indo-1, while cells were held under voltage clamp using patch clamp techniques. 2. Depolarization of cells evoked a transient rise in Ca2+i that increased with increasing duration of depolarization to a peak at about 10 s. 3. Calcium transients showed a bell-shaped dependence on the amplitude of the depolarizing pulse. They were abolished in the absence of extracellular calcium and by application of 10 microM-nifedipine. 4. Thyrotrophin-releasing hormone (TRH) evoked a transient rise in Ca2+i that was followed by a more sustained period of elevated Ca2+i in some cells. The transient phase of the response but not the sustained phase was seen in the absence of extracellular calcium. 5. Ca2+i transients evoked by depolarization were not affected by pre-release of internal Ca2+ stores with TRH. 6. The results demonstrate that voltage-gated Ca2+ entry and Ca2+ store release can each elevate cytoplasmic free calcium in GH3 cells and may both be important for stimulus-secretion coupling. Non-voltage-gated Ca2+ entry is not a major source of Ca2+ under these conditions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1189171Documentos Relacionados
- Voltage-gated Ca2+ channel in mouse myeloma cells.
- Characteristics of voltage-gated Ca2+ currents in ovine gonadotrophs.
- Simulation of Spontaneous Ca2+ Oscillations in Astrocytes Mediated by Voltage-Gated Calcium Channels
- Mechanosensitivity of voltage-gated calcium currents in rat anterior pituitary cells.
- A Homolog of Voltage-Gated Ca2+ Channels Stimulated by Depletion of Secretory Ca2+ in Yeast