Wheat nuclear protein HBP-1 binds to the hexameric sequence in the promoter of various plant genes.

AUTOR(ES)

Mikami, K

RESUMO

HBP-1 is a sequence-specific DNA-binding protein that interacts with the hexameric sequence ACGTCA, the putative cis-acting element of the wheat histone H3 gene. Gel mobility shift and DNase I footprint analyses showed that this protein interacts with homologous sequences in the regulatory regions for the transcription of the cauliflower mosaic virus (CaMV) 35S RNA and nopaline synthase (NOS) genes, evidence that HBP-1 may bind to hexameric sequences in the regulatory regions of various genes. An HBP-1-like protein, indistinguishable from wheat HBP-1 in its the DNA-binding specificity, is present in sunflower nuclear extract, an indication that HBP-1-like DNA-binding proteins also exist in dicots.

Documentos Relacionados

• Interleukin 6 induces a liver-specific nuclear protein that binds to the promoter of acute-phase genes.
• The purification of an erythroid protein which binds to enhancer and promoter elements of haemoglobin genes.
• Phylogenetic footprinting reveals a nuclear protein which binds to silencer sequences in the human gamma and epsilon globin genes.
• Modification of the coding sequence enhances plant expression of insect control protein genes.
• An alternative protein factor which binds the internal promoter of Xenopus 5S ribosomal RNA genes.