Whole blood assay to access T cell-immune responses to Mycobacterium tuberculosis antigens in healthy Brazilian individuals

AUTOR(ES)

Antas, Paulo RZ, Cardoso, Fernando LL, Oliveira, Eliane B, Gomes, Patrícia KC, Cunha, Kátia S, Sarno, Euzenir N, Sampaio, Elizabeth P

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2004-02

RESUMO

The production of interferon gamma (IFNgamma) guarantees effective T cell-mediated immunity against Mycobacterium tuberculosis infection. In the present study, we simply compare the in vitro immune responses to Mycobacterium antigens in terms of IFNg production in a total of 10 healthy Brazilian volunteers. Whole blood and mononuclear cells were cultivated in parallel with PPD, Ag85B, and M. bovis hsp65, and five-days supernatants were harvested for cytokine detection by ELISA. The inter-assay result was that the overall profile of agreement in response to antigens was highly correlated (r² = 0.9266; p = 0.0102). Potential analysis is in current progress to dictate the usefulness of this method to access the immune responses also in tuberculosis patients and its contacts.

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