Axenic Amastigotes
Mostrando 1-12 de 19 artigos, teses e dissertações.
-
1. In vitro evaluation of the anti-leishmanial activity and toxicity of PK11195
BACKGROUND Leishmaniasis, one of the most neglected diseases, is a serious public health problem in many countries, including Brazil. Currently available treatments require long-term use and have serious side effects, necessitating the development of new therapeutic interventions. Because translocator protein (TSPO) levels are reduced in Leishmania amazonen
Mem. Inst. Oswaldo Cruz. Publicado em: 05/02/2018
-
2. Dipyridamole potentiated the trypanocidal effect of nifurtimox and improved the cardiac function in NMRI mice with acute chagasic myocarditis
BACKGROUND As chronic Chagas disease does not have a definitive treatment, the development of alternative therapeutic protocols is a priority. Dipyridamole (DPY) is an alternative to counteract the pathophysiological phenomena involved in Chagas cardiomyopathy. OBJECTIVE To evaluate the therapeutic efficacy of DPY associated with nifurtimox (Nfx) in epim
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-09
-
3. Therapeutic switching: from antidermatophytic essential oils to new leishmanicidal products
This study examined whether the antidermatophytic activity of essential oils (EOs) can be used as an indicator for the discovery of active natural products against Leishmania amazonensis. The aerial parts of seven plants were hydrodistilled. Using broth microdilution techniques, the obtained EOs were tested against three strains of dermatophytes (Trichophyto
Mem. Inst. Oswaldo Cruz. Publicado em: 13/02/2015
-
4. In vitro evaluation of new terpenoid derivatives against Leishmania infantum and Leishmania braziliensis
The activity of five (1-5) abietane phenol derivatives against Leishmania infantum and Leishmania braziliensis was studied using promastigotes and axenic and intracellular amastigotes. Infectivity and cytotoxicity tests were performed with J774.2 macrophage cells using Glucantime as a reference drug. The mechanisms of action were analysed by performing metab
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-05
-
5. Avaliação do potencial antileishmania dos compostos naturais isolados ácido úsnico, cumarin, quercetina e reserpina sobre as formas promastigotas e amastigotas de Leishmania Chagasi
As leishmanioses constituem-se por um complexo de doenças infectoparasitárias, causadas por parasitas do gênero Leishmania, e apresentam um espectro de sintomas variando de simples lesões cutâneas de cicatrização espontânea a lesões viscerais letais. A leishmaniose visceral, causada pela espécie Leishmania chagasi, no Brasil infecta cerca de duas m
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/08/2008
-
6. Morphological comparison of axenic amastigogenesis of trypomastigotes and metacyclic forms of Trypanosoma cruzi
Amastigogenesis occurs first when metacyclic trypomastigotes from triatomine urine differentiate into amastigotes inside mammalian host cells and a secondary process when tissue-derived trypomastigotes invade new cells and differentiate newly to amastigotes. Using scanning electron microscopy, we compared the morphological patterns manifested by trypomastigo
Memórias do Instituto Oswaldo Cruz. Publicado em: 2003-01
-
7. Production of amastigotes from metacyclic trypomastigotes of Trypanosoma cruzi
Attempts to recreate all the developmental stages of Trypanosoma cruzi in vitro have thus far been met with partial success. It is possible, for instance, to produce trypomastigotes in tissue culture and to obtain metacyclic trypomastigotes in axenic conditions. Even though T. cruzi amastigotes are known to differentiate from trypomastigotes and metacyclic t
Memórias do Instituto Oswaldo Cruz. Publicado em: 2002-12
-
8. Identification of a differentially expressed mRNA in axenic Leishmania panamensis amastigotes
Differential display technique was applied in order to identify transcripts which are present in axenic amastigotes but not in promastigotes of the Leishmania panamensis parasites. One of them was cloned and the sequence reveals an open reading frame of 364 amino acids (aprox. 40 kDa). The deduced protein is homologous to the serine/threonine protein kinases
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-08
-
9. DNA Transformation of Leishmania infantum Axenic Amastigotes and Their Use in Drug Screening
Protocols for DNA electroporation in Leishmania promastigote cells are well established. More recently, in vitro culture of axenic Leishmania amastigotes became possible. We have established conditions for DNA transformation of axenically grown Leishmania infantum amastigotes. Parameters for DNA electroporation of Leishmania axenic amastigotes were systemati
American Society for Microbiology.
-
10. An axenic amastigote system for drug screening.
Currently available primary screens for selection of candidate antileishmanial compounds are not ideal. The choices include screens that are designed to closely reflect the situation in vivo but are labor-intensive and expensive (intracellular amastigotes and animal models) and screens that are designed to facilitate rapid testing of a large number of drugs
-
11. Pentostam induces resistance to antimony and the preservative chlorocresol in Leishmania donovani promastigotes and axenically grown amastigotes.
An axenic amastigote culture system was utilized to directly assess the stage-specific antileishmanial effects of antimony on amastigotes of Leishmania donovani devoid of the macrophage host cell. Pentostam, which contains antimony in the form of sodium stibogluconate and the preservative chlorocresol, was used. Cell density was quantified by measuring the a
-
12. Antimonial-Mediated DNA Fragmentation in Leishmania infantum Amastigotes
The basic treatment of leishmaniasis consists in the administration of pentavalent antimonials. The mechanisms that contribute to pentavalent antimonial toxicity against the intracellular stage of the parasite (i.e., amastigote) are still unknown. In this study, the combined use of several techniques including DNA fragmentation assay and in situ and cytofluo
American Society for Microbiology.