Bioluminescent Bacterial Biosensor
Mostrando 1-4 de 4 artigos, teses e dissertações.
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1. ASSESSMENT OF MERCURY (II) SPECIES BIOAVAILABILITY USING A BIOLUMINESCENT BACTERIAL BIOSENSOR
Mercury and its compounds are highly toxic, especially methylmercury - a potent neurotoxin. It has caused a significant number of human fatalities in several accidents around the world. Due to its wide dispersion through the atmosphere, mercury is considered a global pollutant, being deposited even in remote pristine aquatic systems, where it is biomagnified
Publicado em: 2003
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2. Measurement of Biologically Available Naphthalene in Gas and Aqueous Phases by Use of a Pseudomonas putida Biosensor
Genetically constructed microbial biosensors for measuring organic pollutants are mostly applied in aqueous samples. Unfortunately, the detection limit of most biosensors is insufficient to detect pollutants at low but environmentally relevant concentrations. However, organic pollutants with low levels of water solubility often have significant gas-water par
American Society for Microbiology.
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3. Study of the Response of a Biofilm Bacterial Community to UV Radiation
We have developed a bioluminescent whole-cell biosensor that can be incorporated into biofilm ecosystems. RM4440 is a Pseudomonas aeruginosa FRD1 derivative that carries a plasmid-based recA-luxCDABE fusion. We immobilized RM4440 in an alginate matrix to simulate a biofilm, and we studied its response to UV radiation damage. The biofilm showed a protective p
American Society for Microbiology.
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4. Transcription of the Escherichia coli fliC gene is regulated by metal ions.
luxAB gene fusions in the Escherichia coli genome were used to screen for clones displaying transcriptional changes in the presence of aluminum. One clone was found that contained a luciferase gene fusion in which transcription was increased in the presence of aluminum and which was subsequently shown to be induced by copper, iron, and nickel. Cloning of the