Bpde
Mostrando 1-12 de 35 artigos, teses e dissertações.
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1. Avaliação do efeito quimioprotetor de B-glucanas de origem fúngica e vegetal no DNA de células eucararióticas
As ß-glucanas são polissacarídeos extraídos da parede celular de diversos organismos. Composto este que atraiu a atenção devido a suas propriedades medicinais. O objetivo do presente estudo foi de testar o efeito genotóxico e ou antigenotóxico de BG extraídas de Agaricus blazei e cevada, contra agentes genotóxicos diretos (Metil metanosulfonato -MM
Publicado em: 2007
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2. Influencia dos polimorfismos nos genes CYP1A1*2A, GSTM1, GSTT1 e GSTP1*B na susceptibilidade ao cancer de pulmão / Influence of the polymorphisms CYP1A1*2A, GSTM1, GSTT1 e GSTP1*B genes on lung cancer susceptibility
Cytochrome P-450 A1 (CYP1A1 ) is an important enzyme of carcinogen metabolism. Due to polymorphic regulation, CYP1A1 is a biomarker of genetic susceptibility to certain malignancies, especially lung cancer (LC). The first detected variation, named m1 or *2A, was a T-to-C transition in exon 7, with 1194bp, which produced a breakage point for the MspI enzyme.
Publicado em: 2007
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3. Solid-Phase purification of deoxyguanosine-benzo[a]pyrene diol epoxide adducts from genomic DNA adduct synthesis
Polycyclic aromatic hydrocarbons are compounds widely present in the environment and well known to have carcinogenic and/or mutagenic properties. These substances when present in an organism are metabolized and can bind to DNA forming an adduct. Such adduct can induce replication errors that may cause carcinogenic tumor or genetic mutation. As a consequence,
Journal of the Brazilian Chemical Society. Publicado em: 2005-08
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4. dGMP-BPDE DNA adduct investigation in environmentally exposed rural workers by capillary electrophoresis with laser-induced fluorescence detection
Um método alternativo à pos-marcação com 32P radioativo foi proposta para a determinação do aduto de desoxiguanosina monofosfato com epóxido de benzo[a]pirenodiol (dGMP-BPDE), um biomarcador para exposição humana à hidrocarbonetos policíclicos aromáticos (PAH) carcinogênicos, usando eletroforese capilar com fluorescência induzida a laser (CE-LI
Journal of the Brazilian Chemical Society. Publicado em: 2005-04
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5. Differences in unwinding of supercoiled DNA induced by the two enantiomers of anti-benzo[a]pyrene diol epoxide.
The unwinding of supercoiled phi X174 RFI DNA induced by the tumorigenic (+) and non-tumorigenic (-) enantiomers of trans-7,8-dihydroxy-anti-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) has been investigated by agarose slab-gel and ethidium titration tube gel electrophoresis. The differences in adduct conformations were verified by flow linear dichroi
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6. Stereochemistry-dependent bending in oligonucleotide duplexes induced by site-specific covalent benzo[a]pyrene diol epoxide-guanine lesions.
The apparent persistence length of enzymatically linearized pIBI30 plasmid DNA molecules approximately 2300 bp long, as measured by a hydrodynamic linear flow dichroism method, is markedly decreased after covalent binding of the highly tumorigenic benzo[a]pyrene metabolite 7R,8S-dihydroxy-9S,10R-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(+)-anti-BPDE]. In str
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7. Inhibition of DNA synthesis by an electrophilic metabolite of benzo[a]pyrene.
Mitogen-stimulated scheduled DNA synthesis and DNA excision repair in human lymphocytes, as well as DNA polymerase a activity in a cell-free system, were inhibited by an electrophilic metabolite of benzo[a]pyrene. This metabolite, (+/-)-anti-(7r,8t)-dihydroxy-(9,10t)-epoxy-7,8,9,10-tetrahyd robenzo[a]pyrene (BPDE), covalently binds to cellular macromolecules
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8. Base pair conformation-dependent excision of benzo[a]pyrene diol epoxide-guanine adducts by human nucleotide excision repair enzymes.
Human nucleotide excision repair processes carcinogen-DNA adducts at highly variable rates, even at adjacent sites along individual genes. Here, we identify conformational determinants of fast or slow repair by testing excision of N2-guanine adducts formed by benzo[a]pyrene diol epoxide (BPDE), a potent and ubiquitous mutagen that induces mainly G x C-->T x
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9. Dose-dependent differences in the profile of mutations induced by an ultimate carcinogen from benzo[a]pyrene.
Mutations in the coding region of the hypoxanthine (guanine) phosphoribosyltransferase (HPRT) gene of Chinese hamster V-79 cells were examined after exposure of the cells to a high cytotoxic dose (0.48 microM; 35% survival) and a low noncytotoxic dose (0.04 microM; 100% survival) of the ultimate carcinogen (+)-7R,8S-dihydroxy-9S,10R-epoxy-7,8,9,10-tetrahydro
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10. Preferential repair and strand-specific repair of benzo[a]pyrene diol epoxide adducts in the HPRT gene of diploid human fibroblasts.
If excision repair-proficient human cells are allowed time for repair before onset of S phase, the premutagenic lesions formed by (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy- 7,8,9,10-tetrahydrobenzo[a]pyrene (benzo[a]pyrene diol epoxide, BPDE) are lost from the transcribed strand of the hypoxanthine (guanine) phosphoribosyltransferase (HPRT) gene
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11. Kinds of mutations formed when a shuttle vector containing adducts of benzo[a]pyrene-7,8-diol-9,10-epoxide replicates in COS7 cells.
We have investigated the kinds of mutations induced when a shuttle vector containing covalently bound residues of the (+/-)-7 beta,8 alpha-dihydroxy-9 alpha,10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) replicates in the monkey kidney cell line COS7. The target for detecting mutations was the 200-base pair gene for a tyrosine suppressor tRNA (supF)
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12. Site-specific carcinogen binding to DNA.
Benzo[alpha]pyrene diol epoxide (BPDE) is a well-studied environmental carcinogen that binds covalently to DNA. Here we describe a photochemical technique that allows us to map BPDE-binding sites within cloned gene sequences. The technique is based upon our observation that, when irradiated with laser light at 355 nm, one single-strand DNA cut is produced at