Cationic Amphiphiles
Mostrando 1-10 de 10 artigos, teses e dissertações.
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1. Partículas e filmes híbridos de polímeros e compostos de amônio quaternário com atividade antimicrobiana / Particles and films hybrid of polymers and quaternary ammonium compounds with antimicrobial activity
Partículas e filmes híbridos de polímeros e compostos de amônio quaternário (CAQ) foram caracterizados quanto a propriedades físicas e atividade contra cepas de Pseudomonas aeruginosa ATCC 27853 e Staphylococcus aureus ATCC 25923. Partículas híbridas foram obtidas a partir de fragmentos de bicamada (BF) de brometo de dioctadecildimetilamônio (DODAB)
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 02/02/2011
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2. Cholesterol-induced conformational change in SCAP enhanced by Insig proteins and mimicked by cationic amphiphiles
Sterols mediate feedback inhibition of the sterol regulatory element-binding protein (SREBP) pathway by preventing movement of the SREBP cleavage-activating protein (SCAP)/SREBP complex from endoplasmic reticulum (ER) to Golgi, where proteolytic cleavage of SREBPs releases the transcription factor domain that activates genes for lipid biosynthesis. Our labor
National Academy of Sciences.
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3. Cationic facial amphiphiles: a promising class of transfection agents.
A promising class of compounds for DNA transfection have been designed by conjugating various polyamines to bile-acid-based amphiphiles. Formulations containing these compounds were tested for their ability to facilitate the uptake of a beta-galactosidase reporter plasmid into COS-7 cells. Dioleoyl phosphatidyl ethanolamine (DOPE) formulations of some of the
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4. Novel cationic amphiphiles as delivery agents for antisense oligonucleotides.
There has been great interest recently in therapeutic use of nucleic acids including genes, ribozymes and antisense oligonucleotides. Despite recent improvements in delivering antisense oligonucleotides to cells in culture, nucleic acid-based therapy is still often limited by the poor penetration of the nucleic acid into the cytoplasm and nucleus of cells. I
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5. Compaction of DNA in an anionic micelle environment followed by assembly into phosphatidylcholine liposomes
A difficult problem concerning the interaction of DNA with amphiphiles of opposite charge above their critical micelle concentration is the propensity for aggregation of the condensed DNA complexes. In this study, this problem was addressed by attenuating amphiphile charge density within a cholate micelle environment. The amphiphile consisted of a cationic p
Oxford University Press.
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6. Monomolecular collapse of plasmid DNA into stable virus-like particles
Cationic lipids are being widely used for cell transfection in vitro. The lipid/DNA complexes, however, tend to aggregate into large and polydisperse particle mixtures; this hampers their use in vivo. Cationic detergents, on the contrary, do not mediate cell transfection per se, yet are capable of condensing individual DNA molecules into discrete entities. W
The National Academy of Sciences.
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7. Effects of polycations on pulmonary vascular permeability in conscious sheep.
The role of charged sites on the permeability characteristics of the pulmonary microvascular barrier were investigated using chronically instrumented unanesthetized sheep. In one series of experiments we studied the effects of the cationic amphiphile, dodecyl trimethylamine (DTA; 297 mol wt), and the anionic amphiphile, SDS (288 mol wt), on lung lymph flow r
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8. On the osmotic signal and osmosensing mechanism of an ABC transport system for glycine betaine
The osmosensing mechanism of the ATP-binding cassette (ABC) transporter OpuA of Lactococcus lactis has been elucidated for the protein reconstituted in liposomes. Activation of OpuA by osmotic upshift was instantaneous and reversible and followed changes in volume and membrane structure of the proteoliposomes. Osmotic activation of OpuA was dependent on the
Oxford University Press.
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9. Novel pyridinium surfactants for efficient, nontoxic in vitro gene delivery
Novel, double-chained pyridinium compounds have been developed that display highly efficient DNA transfection properties. The transfection efficiency of several of these compounds is enhanced by an order of magnitude, when compared with the transfection efficiency accomplished with the widely used cationic lipid system, lipofectin. Most importantly, the pyri
The National Academy of Sciences of the USA.
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10. Adenosine triphosphate-dependent transport of doxorubicin, daunomycin, and vinblastine in human tissues by a mechanism distinct from the P-glycoprotein.
Previous studies have demonstrated that a human glutathione conjugate transporter, designated as dinitrophenyl-S-glutathione ATPase (DNP-SG ATPase), catalyzed ATP hydrolysis in the presence of several amphiphilic compounds other than glutathione conjugates (Singhal, S. S., R. Sharma, S. Gupta, H. Ahmad, P. Zimniak, A. Radominska, R. Lester, and Y. C. Awasthi