Cdna Libraries
Mostrando 1-12 de 670 artigos, teses e dissertações.
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1. Transcriptome sequencing and expression profiling of genes involved in the response to abiotic stress in Medicago ruthenica
Abstract Medicago ruthenica is a perennial forage legume with the remarkable ability to survive under unfavorable environmental conditions. It has been identified as an excellent species of Medicago that can adapt to various environmental stresses including low temperature, drought, and salinity. To investigate its potential as a genetic resource, we perform
Genet. Mol. Biol.. Publicado em: 28/06/2018
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2. Insights into the dynamics of hind leg development in honey bee (Apis mellifera L.) queen and worker larvae - A morphology/differential gene expression analysis
Phenotypic plasticity is a hallmark of the caste systems of social insects, expressed in their life history and morphological traits. These are best studied in bees. In their co-evolution with angiosperm plants, the females of corbiculate bees have acquired a specialized structure on their hind legs for collecting pollen. In the highly eusocial bees (Apini a
Genet. Mol. Biol.. Publicado em: 21/08/2015
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3. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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4. A web-based bioinformatics interface applied to the GENOSOJA project: databases and pipelines
The Genosoja consortium is an initiative to integrate different omics research approaches carried out in Brazil. Basically, the aim of the project is to improve the plant by identifying genes involved in responses against stresses that affect domestic production, like drought stress and Asian Rust fungal disease. To do so, the project generated several types
Genetics and Molecular Biology. Publicado em: 2012
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5. Transcriptome analysis of resistant soybean roots infected by Meloidogyne javanica
Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the trans
Genet. Mol. Biol.. Publicado em: 2012
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6. Caracterização funcional de dois cDNAs homólogos à ciclofilina e à proteína reprimida por auxina (ARP) identificados em bibliotecas subtrativas a para floração em tomateiro
A floração é fundamental no ciclo de vida de uma planta. Este processo é marcado pela conversão do meristema apical vegetativo em reprodutivo, devido a interações entre diversos fatores, tanto internos quanto externos à planta. Diante disso, foram construídas oito bibliotecas subtrativa utilizando ápices meristemáticos induzidos e não induzidos p
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 09/09/2011
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7. Differentially expressed genes and miRNA identification in pig skeletal muscle / Identificação de genes diferencialmente expressos e miRNAs em músculo esquelético de suínos
The pig (Sus scrofa) is considered an important animal for meat production. This interest revolves around the potential for muscle growth, which usually is associated with certain characteristics during prenatal myogenesis. To study the genes responsible for these characteristics, expressed sequence tags (EST) provide direct information about the transcripto
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 25/07/2011
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8. Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expr
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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9. Aluminum-induced genes in grass species.
This study aimed to identify genes associated with Al tolerance mechanisms in grasses, using cDNA and subtracitve libraries derived from roots of maize, rice, sorghum, oat, barley, wheat and brachiaria tolerant genotypes submitted to critical levels of Al in nutrient solution.
INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY. Publicado em: 2011
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10. Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms.
Three cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205
Planta. Publicado em: 2011
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11. Aluminum-induced genes in grass species.
The toxicity caused by aluminum (Al), intrinsic to acid soils, in?uences negatively the stability of crop production. Under toxic levels of Al, plant roots paralyze their development and become unable to explore the deeper layers of the soil, affecting nutrient and water acquisition and reducing crop yield. This study aimed to identify genes associated with
INTERNATIONAL CONFERENCE OF THE BRAZILIAN ASSOCIATION FOR BIOINFORMATICS AND COMPUTATIONAL BIOLOGY. Publicado em: 2011
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12. Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH)
Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previou
Genetics and Molecular Biology. Publicado em: 2010