Centrosomes
Mostrando 1-12 de 140 artigos, teses e dissertações.
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1. Novel insights into the genetic and epigenetic paternal contribution to the human embryo
The integrity of the sperm genome and epigenome are critical for normal embryonic development. The advent of assisted reproductive technology has led to an increased understanding of the role of sperm in fertilization and embryogenesis. During fertilization, the sperm transmits not only nuclear DNA to the oocyte but also activation factor, centrosomes, and a
Clinics. Publicado em: 2013
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2. Estudos estruturais e funcionais das proteínas cinases humanas Nek1 e Nek6 / Structural and functional studies of Nek1 Nek6 protein kinases
NIMA was identified and functionally characterized in Aspergillus nidulans as a critical Ser/Thr kinase for cell cycle progression. The mammalian Neks (NIMA-related kinases) represent an evolutionarily conserved family of 11 serine/threonine kinases that share 40-45% identity with NIMA N-terminal domain. Neks are associated to cell cyclerelated functions and
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 04/03/2011
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3. The study of biological meaning of multinucleation induced by vincristine in cultured cells / Estudo do significado biológico da multinucleação induzida por vincristina em células em cultura
The study of agents that interfere in the functionality of proteins related to cell cycle is important for the understanding of the transformation and cell death processes. Although ploidy alterations are presented in the majority of human tumors, their role in oncogenic process is not understood yet. The alteration on chromosomal number is the primary conse
Publicado em: 2006
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4. Identification of genes coding for serine/threonine phosphatases in Dictyostelium discoideum and functional characterization of type 4 protein phosphatase (PP4) / Identificação de genes codificadores de serina/treonina fosfatases em Dictyostelium discoideum e caracterização funcional da proteína fosfatase do tipo 4 (PP4)
The serine/threonine protein phosphatases (PPs) are enzymes responsible for dephosphorylation of phosphoserine and/or phosphothreonine residues and are divided in two gene families designated as PPP and PPM. The PPP family is divided in five subfamilies, which comprise type 1 (PP1), 2A (PP2A), 2B (PP2B), 5 (PP5) and 7 (PP7) phosphatases. This subdivision is
Publicado em: 2003
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5. Differential regulation of maternal vs. paternal centrosomes
Centrosomes are the main microtubule-organizing centers in animal cells. During meiosis and mitosis, two centrosomes form the poles that direct the assembly of a bipolar spindle, thus ensuring the accurate segregation of chromosomes. Cells cannot tolerate the presence of more than two active centrosomes during meiosis or mitosis because doing so results
The National Academy of Sciences.
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6. Behavior of centrosomes during fertilization and cell division in mouse oocytes and in sea urchin eggs.
The forms and locations of centrosomes in mouse oocytes and in sea urchin eggs were followed through the whole course of fertilization and first cleavage by immunofluorescence microscopy. Centrosomes were identified with an autoimmune antiserum to centrosomal material. Staining of the same preparations with tubulin antibody and with the DNA dye Hoechst 33258
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7. Centrosomes competent for parthenogenesis in Xenopus eggs support procentriole budding in cell-free extracts.
Heterologous centrosomes from diversed species including humans promote egg cleavage when injected into metaphase-arrested Xenopus eggs. We have recently isolated centrosomes from calf thymocytes and shown that they were unable to induce egg cleavage, an inability that was apparently correlated with the peculiar structure of these centrosomes rather than wit
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8. Involvement of Crm1 in Hepatitis B Virus X Protein-Induced Aberrant Centriole Replication and Abnormal Mitotic Spindles
Hepatitis B virus (HBV) includes an X gene (HBx gene) that plays a critical role in liver carcinogenesis. Because centrosome abnormalities are associated with genomic instability in most human cancer cells, we examined the effect of HBx on centrosomes. We found that HBx induced supernumerary centrosomes and multipolar spindles. This effect was independent of
American Society for Microbiology.
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9. Outer Dense Fiber 2 Is a Widespread Centrosome Scaffold Component Preferentially Associated with Mother Centrioles: Its Identification from Isolated Centrosomes
Because centrosomes were enriched in the bile canaliculi fraction from the chicken liver through their association with apical membranes, we developed a procedure for isolation of centrosomes from this fraction. With the use of the centrosomes, we generated centrosome-specific monoclonal antibodies. Three of the monoclonal antibodies recognized an antig
The American Society for Cell Biology.
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10. Centrosomes Split in the Presence of Impaired DNA Integrity during MitosisV⃞
A well-established function of centrosomes is their role in accomplishing a successful mitosis that gives rise to a pair of identical daughter cells. We recently showed that DNA replication defects and DNA damage in Drosophila embryos trigger centrosomal changes, but it remained unclear whether comparable centrosomal responses can be provoked in somatic
The American Society for Cell Biology.
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11. p53 Localization at Centrosomes during Mitosis and Postmitotic Checkpoint Are ATM-dependent and Require Serine 15 Phosphorylation
We recently demonstrated that the p53 oncosuppressor associates to centrosomes in mitosis and this association is disrupted by treatments with microtubule-depolymerizing agents. Here, we show that ATM, an upstream activator of p53 after DNA damage, is essential for p53 centrosomal localization and is required for the activation of the postmitotic checkpoint
The American Society for Cell Biology.
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12. Translocation of XRCC1 and DNA ligase IIIα from centrosomes to chromosomes in response to DNA damage in mitotic human cells
DNA single-strand breaks (SSBs) are the most frequent lesions caused by oxidative DNA damage. They disrupt DNA replication, give rise to double-strand breaks and lead to cell death and genomic instability. It has been shown that the XRCC1 protein plays a key role in SSBs repair. We have recently shown in living human cells that XRCC1 accumulates at SSBs in a
Oxford University Press.