Chemostat Culture
Mostrando 1-12 de 199 artigos, teses e dissertações.
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1. Cultura de células de Drosophila melanogaster (S2) em processo contínuo. / Culture of Drosophila melagogaster cells (S2) in continuous culture.
As células de Drosophila melanogaster (S2) têm sido utilizadas como sistemas de expressão de proteínas recombinantes. Neste trabalho foi utilizada uma linhagem S2 geneticamente modificada com vetores de expressão para a produção da glicoproteína do vírus da raiva (GPV). O principal objetivo deste trabalho foi avaliar o comportamento destas células
Publicado em: 2010
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2. Processo contínuo de produção de polihidroxialcanoatos de cadeia média (PHAMCL) sob limitação múltipla de nutrientes. / Medium-chain-length polyhydroxylkanoates production in chemostat culture under multiple nutrient limitation.
A produção de PHAMCL por Pseudomonas putida IPT 046 em cultivo contínuo sob limitação múltipla de nutrientes foi estudada, utilizando glicose e frutose como fontes de carbono. O estudo da limitação em nitrogênio, fósforo e simultânea de nitrogênio e fósforo para indução de acúmulo de polihidroxialcanoato, apontou que maiores valores de polím
Publicado em: 2008
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3. Effect of phosphate on the multiplication of Saccharomyces cerevisiae in continuous cultivation / Efeito de fosfato sôbre a multiplicação de Saccharomyces cerevisiae em cultivo contínuo
The influence of phosphate in a continuous culture was studied using mini-fermentor on the Saccharomyces cerevisiae multiplication. Dry matter, substrate concentration, specific substrate comsumption, specific carbon dioxide release, specific oxygen uptake rates and respiration quotient , as well as phosphorous content of the cells were measured in dependenc
Publicado em: 1983
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4. Production and localization of beta-fructosidase in asynchronous and synchronous chemostat cultures of yeasts.
In synchronized continuous cultures of Saccharomyces cerevisiae CBS 8066, the production of the extracellular invertase (EC 3.2.1.26) showed a cyclic behavior that coincided with the budding cycle. The invertase activity increased during bud development and ceased at bud maturation and cell scission. The cyclic changes in invertase production resulted in cyc
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5. Microbial Ecophysiology of Whey Biomethanation: Comparison of Carbon Transformation Parameters, Species Composition, and Starter Culture Performance in Continuous Culture
Changes in lactose concentration and feed rate altered bacterial growth and population levels in a whey-processing chemostat. The bacterial population and methane production levels increased in relation to increased lactose concentrations comparable to those in raw whey (6%) and converted over 96% of the substrate to methane, carbon dioxide, and cells. Seque
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6. Chemostat Culture of Escherichia coli K-12 Limited by the Activity of Alkaline Phosphatase
The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of β-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is dis
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7. Characteristics of the constituent substrains of Bacillus popilliae growing in batch and continuous cultures.
Continuous culture of Bacillus popilliae was achieved for the first time in a small chemostat. Initially, variable cell yields during steady-state chemostat growth led to a re-examination of growth rates in batch cultures. B. popilliae NRRL B-2309 and a wild strain were both found to be natural mixtures of three substrains characterized by different growth r
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8. Strain selection in carbon-limited chemostats affects reproducibility of Thermoanaerobacter ethanolicus fermentations.
We found that the reproducibility of chemostat trials can be improved by using chemostat-adapted strains. Our experimental findings are consistent with adaptation that involves an improvement in culture fitness and an alteration of the fermentation genotype.
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9. Removal of Cadmium by Microorganisms in a Two-Stage Chemostat
A two-stage chemostat was used to study removal of cadmium by microorganisms in continuous culture. The medium was contaminated with 0.8 mg of Cd per liter. At 20°C, most of the microbial biomass formed aggregates which settled in the second stage of the chemostat. Effluent was free of bacteria. Up to 80% of the metal contained in the inlet flux was removed
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10. Parathion utilization by bacterial symbionts in a chemostat.
A continuous-culture device was used to select and enrich for microorganisms, from sewage and agricultural runoff, that were capable of using the organophosphorus insecticide parathion as a sole growth substrate. Parathion was dissimilated by the highly acclimated symbiotic activities of Pseudomonas stutzeri, which non-oxidatively and cometabolically hydroly
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11. Coevolution of Bacteriophage PP01 and Escherichia coli O157:H7 in Continuous Culture
The interaction between Escherichia coli O157:H7 and its specific bacteriophage PP01 was investigated in chemostat continuous culture. Following the addition of bacteriophage PP01, E. coli O157:H7 cell lysis was observed by over 4 orders of magnitude at a dilution rate of 0.876 h−1 and by 3 orders of magnitude at a lower dilution rate (0.327 h−1). Howeve
American Society for Microbiology.
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12. Growth Conditions and Rifampin Susceptibility
The susceptibility of Escherichia coli to rifampin was measured during unlimited growth in rich and poor media and during chemostat growth limited by the carbon source. During batch growth at low turbidities, the susceptibility of the bacteria increased as the growth rate decreased, consistent with the longer time available for drug penetration in the poorer