Chitin Synthase
Mostrando 1-12 de 71 artigos, teses e dissertações.
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1. Analysis of the Gene Expression and RNAi-Mediated Knockdown of Chitin Synthase from Leaf-Cutting Ant Atta sexdens
Chitin synthase (CHS) is the enzyme specifically associated with chitin synthesis, an important component of diverse organisms including insects. Two alternative spliced transcripts of the CHS gene (AsCHS-A1 and AsCHS-A2) were identified by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) during the development of the leaf-cutting ant A
J. Braz. Chem. Soc.. Publicado em: 2020-10
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2. A case of sporotrichosis caused by different Sporothrix brasiliensis strains: mycological, molecular, and virulence analyses
BACKGROUND Sporotrichosis is a subcutaneous mycosis caused by dimorphic pathogenic fungi belonging to the Sporothrix genus. Pathogenic Sporothrix species typically produce melanin, which is known to be a virulence factor. OBJECTIVES The aim of this study was to perform phenotypic, genotypic, and virulence analyses of two distinct Sporothrix brasiliensis s
Mem. Inst. Oswaldo Cruz. Publicado em: 21/10/2019
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3. Análise da expressão gênica no dermatófito Trichophyton rubrum mimetizando a infecção in vitro: pH e diferentes fontes de carbono regulando genes / Analysis of gene expression in the dermatophyte Trichophyton rubrum during the mimetic infection in vitro: pH and carboun sources are regulating genes
Dermatophytes are a group of fungi filamentous that have the ability to invade keratinized substrates, causing dermatophytosis in humans and animals and only penetrate deeper if the host is immunocompromised. Trichophyton rubrum is an anthropophilic and cosmopolitan fungi, the most common agent of superficial mycoses, which uses cell components such as prote
Publicado em: 2008
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4. $a Síntese de derivados da D-arabinose e investigação da sua atividade antifúngica
The glucosamine-6-phosphate synthase is the enzyme that catalyses the conversion of D-frutose-6-phosphate to D-glucosamine-6-phosphate. This is the first step in the biochemical pathway leading to the formation of an activated form of N-acetyl-Dglucosamine, namely uridine 5-diphospho-N-acetyl-D-glucosamine. This nucleoside provides N-acetyl-D-glucosamine for
Publicado em: 2007
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5. Synthesis, degradation and functions of insect peritrophic membrane / Síntese, degradação e funções da membrana peritrófica dos insetos
A maior parte dos insetos possui uma estrutura anatômica em forma de filme (membrana peritrófica, MP) composta de quitina e proteínas (peritrofinas), que separa o alimento do epitélio do intestino médio. A MP protege o epitélio de microorganismos e da abrasão, e possui outras funções baseadas no fato de que a MP promove a compartimentalização de e
Publicado em: 2005
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6. Nikkomycin Z is a specific inhibitor of Saccharomyces cerevisiae chitin synthase isozyme Chs3 in vitro and in vivo.
Nikkomycin Z inhibits chitin synthase in vitro but does not exhibit antifungal activity against many pathogens. Assays of chitin synthase isozymes and growth assays with isozyme mutants were used to demonstrate that nikkomycin Z is a selective inhibitor of chitin synthase 3. The resistance of chitin synthase 2 to nikkomycin Z in vitro is likely responsible f
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7. Chitin synthase 2 is essential for septum formation and cell division in Saccharomyces cerevisiae.
Previous work led to the puzzling conclusion that chitin synthase 1, the major chitin synthase activity in Saccharomyces cerevisiae, is not required for synthesis of the chitinous primary septum. The mechanism of in vivo synthesis of chitin has now been clarified by cloning the structural gene for the newly found chitin synthase 2, a relatively minor activit
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8. Effects of amphotericin B, nystatin, and other polyene antibiotics on chitin synthase.
The effects of amphotericin B (AmB), nystatin, filipin, and pimaricin were tested chitin synthase (EC 2.4.1.16) (chitosomes from yeast cells of Mucor rouxii). AmB and nystatin inhibited the enzyme at concentrations greater than or equal to 10 micrograms/ml, filipin was weakly inhibitory, and pimaricin had no effect. The inhibition of chitin synthase by AmB a
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9. Localized deposition of chitin on the yeast cell surface in response to mating pheromone
Treatment of a mating-type Saccharomyces cerevisiae cells with the pheromone α-factor (secreted by α mating-type cells) induces the synthesis of chitin. Small daughter cells, which start with no detectable chitin, make 3 times more chitin when grown in the presence of α-factor than do untreated exponentially growing cells. Budding cells accumulate chitin
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10. CSD2, CSD3, and CSD4, genes required for chitin synthesis in Saccharomyces cerevisiae: the CSD2 gene product is related to chitin synthases and to developmentally regulated proteins in Rhizobium species and Xenopus laevis.
In Saccharomyces cerevisiae, chitin forms the primary division septum and the bud scar in the walls of vegetative cells. Three chitin synthetic activities have been detected. Two of them, chitin synthase I and chitin synthase II, are not required for synthesis of most of the chitin present in vivo. Using a novel screen, I have identified three mutations, des
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11. Chitin synthase I and chitin synthase II are not required for chitin synthesis in vivo in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, the polysaccharide chitin forms the primary division septum between mother cell and bud. Two related enzymes, chitin synthase I and chitin synthase II (UDP-acetamido-2-deoxy-D-glucose:chitin 4-beta-acetamidodeoxyglucosyltransferase, EC 2.4.1.16), have been identified and their structural genes, CHS1 and CHS2, respectively, have b
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12. Vectorial synthesis of a polysaccharide by isolated plasma membranes.
To ascertain the directionality of chitin synthesis by yeast plasma membranes, the external surface of Saccharomyces cerevisiae protoplasts was labeled with ferritin--concanavalin A. After protoplast lysis, plasma membranes were isolated and treated with trypsin to activate chitin synthase (UDP-2-acetamido-2-deoxy-D-glucose:chitin 4-beta-acetamidodeoxy-D-glu