Chlorophyllase
Mostrando 1-12 de 14 artigos, teses e dissertações.
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1. RETENÃÃO DE CLOROFILA E SEUS EFEITOS SOBRE A QUALIDADE FISIOLÃGICA DE SEMENTE DE SOJA / Chlorophyll retention and its effects on physiological quality of soybean seed
This study was composed by three experiments related to the effects of chlorophyll retention in soybean seeds. The first one determined the effects of water deficit and high temperature stresses during maturation on the expression level of green seeds. The second evaluated the susceptibility of different genotypes to the expression of this problem. The third
Publicado em: 2006
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2. Substrate Specificity of Chlorophyllase 12
Apparent Km and Vmax values were obtained for hydrolysis of methyl and ethyl chlorophyllides a, methyl and ethyl pheophorbide a, and 9-hydroxymethyl pheophorbide a by chlorophyllase from Ailanthus altissima. Analysis of substrate specificity data for chlorophyllase indicates that the presence of a 9-keto group and a methyl alcohol group esterified at the 7-p
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3. Sequence of Chloroplast Degreening in Calamondin Fruit as Influenced by Ethylene and AgNO31
C2H4 disrupts the internal membranes of the chloroplast and induces an increase in chlorophyllase activity in degreening calamondin [x Citrofortunella mitis (Blanco) Ingram and Moore] fruit. Whether the loss of chlorophyll in the peel is causally related to breakdown of the chloroplast and/or chlorophyllase activity is not readily apparent. Chlorophyllase le
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4. Involvement of Ethylene in Chlorophyll Degradation in Peel of Citrus Fruits 1
The effect of ethylene on chlorophyll degradation in the peel of Robinson tangerine (X Citrus reticulata Blanco) and calamondin (X Citrofortunellamitis [Blanco] Ingram and Moore) fruits was studied. The chlorophyll degrading system in the peel of these two citrus species was not self-sustaining but required ethylene to function. Chlorophyll degradation cease
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5. Chlorophyllase in Soybean. 123
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6. Chlorophyllase Activity of Spinach Chloroplastin 12
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7. Chlorophyllase and Formation of an Atypical Chlorophyllide in Marine Algae 1
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8. Accumulation of α-Tocopherol in Senescing Organs as Related to Chlorophyll Degradation
α-Tocopherol (α-T) has been identified, using gas chromatography-mass spectroscopy and 1H- and 13C-nuclear magnetic resonance, in senescing leaves of Melia azedarach L. The content of α-T increased concomitantly with the breakdown of chlorophyll in senescing Vinca and Melia leaves. An increase in α-T was found also in detached Melia leaves, senescing in
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9. Purification and Properties of Chlorophyllase from Ailanthus altissima (Tree-of-Heaven) 12
Chlorophyllase from Ailanthus altissima leaves has been purified 63-fold by a combination of heat treatment, ultracentrifugation, gel filtration, and chromatography on diethylaminoethyl cellulose. While the enzyme is inhibited to some degree by Triton X-100, a modification of the assay procedure of Klein and Vishniac has been shown to be far superior to the
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10. Chlorophyllase 1, a Damage Control Enzyme, Affects the Balance between Defense Pathways in Plants
Accumulation of reactive oxygen species (ROS) is central to plant response to several pathogens. One of the sources of ROS is the chloroplast because of the photoactive nature of the chlorophylls. Chlorophyllase 1 (encoded by AtCLH1) of Arabidopsis thaliana is quickly induced after tissue damage (e.g., caused by the bacterial necrotroph Erwinia carotovora or
American Society of Plant Biologists.
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11. Cloning of chlorophyllase, the key enzyme in chlorophyll degradation: Finding of a lipase motif and the induction by methyl jasmonate
Chlorophyllase (Chlase) is the first enzyme involved in chlorophyll (Chl) degradation and catalyzes the hydrolysis of ester bond to yield chlorophyllide and phytol. In the present study, we isolated the Chlase cDNA. We synthesized degenerate oligo DNA probes based on the internal amino acid sequences of purified Chlase from Chenopodium album, screened the C.
The National Academy of Sciences.
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12. Ethylene induces de novo synthesis of chlorophyllase, a chlorophyll degrading enzyme, in Citrus fruit peel.
Chlorophyllase (Chlase; EC 3.1.1.14) was extracted from plastid fractions of ethylene-treated orange fruit peel and purified 400-fold to homogeneity by gel filtration, hydrophobic chromatography, and preparative SDS/PAGE of nonheated protein. SDS/PAGE of nonheated purified enzyme indicated that Chlase activity is associated with a single protein band migrati