Clonal Interference
Mostrando 1-12 de 23 artigos, teses e dissertações.
-
1. Virus interference on local scale viticulture: the case of Moll variety from Majorca (Spain)
In Europe, many autochthonous grapevines (Vitis vinifera L.) are only cultivated at local scale, but play a very important economic role due to their strict relation with terroir and wine tipicity. In this study, it was pursued to evaluate the influence of several factors on performance of Moll, the main autochthonous white variety of Majorca, by means of th
Sci. agric. (Piracicaba, Braz.). Publicado em: 2013-04
-
2. Dependência da velocidade da catraca de Muller com fluxo de mutações benéficas : uma avaliação estocástica
Nós investigamos a evolução das populações assexuadas sujeita à ocorrência de um fluxo contínuo de mutações deletérias, onde a Catraca de Muller opera. Neste regime, a acumulação de mutações deletérias ocorre continuamente com a conseqüente perda da classe de indivíduos mais adaptados. Na nossa dissertação nós estudamos o efeito das muta
Publicado em: 2006
-
3. Diminishing Returns of Population Size in the Rate of RNA Virus Adaptation
Whenever an asexual viral population evolves by adapting to new environmental conditions, beneficial mutations, the ultimate cause of adaptation, are randomly produced and then fixed in the population. The larger the population size and the higher the mutation rate, the more beneficial mutations can be produced per unit time. With the usually high mutation r
American Society for Microbiology.
-
4. Absence of Interference During High-Multiplicity Infection by Clonally Purified Vesicular Stomatitis Virus
Stocks of vesicular stomatitis virus free of defective interfering particles were produced by serial clonal isolation. High-multiplicity infections with these stocks led to no interference or formation of defective interfering particles. Defective interfering particles were generated by three successive passages at high multiplicity.
-
5. Clonal Populations of Thermotolerant Enterobacteriaceae in Recreational Water and Their Potential Interference with Fecal Escherichia coli Counts
Bacterial strains were isolated from beach water samples using the original Environmental Protection Agency method for Escherichia coli enumeration and analyzed by pulsed-field gel electrophoresis (PFGE). Identical PFGE patterns were found for numerous isolates from 4 of the 9 days sampled, suggesting environmental replication. 16S rRNA gene sequencing, API
American Society for Microbiology.
-
6. Mitochondrial Development during Life Cycle Differentiation of African Trypanosomes: Evidence for a Kinetoplast-dependent Differentiation Control Point
Life cycle differentiation of African trypanosomes entails developmental regulation of mitochondrial activity. This requires regulation of the nuclear genome and the kinetoplast, the trypanosome's unusual mitochondrial genome. To investigate the potential cross talk between the nuclear and mitochondrial genome during the events of differentiation, we have 1)
The American Society for Cell Biology.
-
7. Human immunodeficiency virus (HIV) type 1 can superinfect HIV-2-infected cells: pseudotype virions produced with expanded cellular host range.
In studies on viral interference, cloned T-cell lines chronically infected with human immunodeficiency virus (HIV) type 1 or HIV-2 were inoculated with several strains of these two AIDS retrovirus subtypes. HIV-2UC1-infected cells, which still express the CD4 receptor, could be superinfected with a variety of HIV-1 and HIV-2 strains. This event was accompani
-
8. Asymmetrical division of the kinetoplast DNA network of the trypanosome
Trypanosome mitochondrial DNA is a network containing thousands of interlocked minicircles. Silencing of a mitochondrial topoisomerase II by RNA interference (RNAi) causes progressive network shrinking, allowing assessment of the minimal network size compatible with viability. We cloned surviving cells after short-term RNAi and found, as expected, that the
Oxford University Press.
-
9. Dominant-negative C/EBP disrupts mitotic clonal expansion and differentiation of 3T3-L1 preadipocytes
Hormonal induction of growth-arrested 3T3-L1 preadipocytes rapidly activates expression of CCAAT/enhancer-binding protein (C/EBP) β. Acquisition of DNA-binding activity by C/EBPβ, however, is delayed until the cells synchronously enter the S phase of mitotic clonal expansion (MCE). After MCE, C/EBPβ activates expression of C/EBPα and peroxisome prolifera
National Academy of Sciences.
-
10. A novel murine oncornavirus with dual eco- and xenotropic properties.
Infection of Swiss mouse 3T3FL cells with a clonal isolate of Moloney leukemia virus (MLV-IC) resulted in virus progeny composed of at least three different murine helper oncornaviruses. Each entity was purified in appropriate cells by several sequential terminal dilution isolations and was grouwn to high titers. Besides ecotropic MLV-IC there was a pure xen
-
11. DNA Methylation of Helper Virus Increases Genetic Instability of Retroviral Vector Producer Cells
Retroviral vector producer cells (VPC) have been considered genetically stable. A clonal cell population exhibiting a uniform vector integration pattern is used for sustained vector production. Here, we observed that the vector copy number is increased and varied in a population of established LTKOSN.2 VPC. Among five subclones of LTKOSN.2 VPC, the vector co
American Society for Microbiology.
-
12. Analysis of the Genetic Variability of Genes Encoding the RNA III-Activating Components Agr and TRAP in a Population of Staphylococcus aureus Strains Isolated from Cows with Mastitis
The expression of Staphylococcus aureus virulence proteins is under the control of RNA III, a central pleiotropic regulator transcribed from the agr locus. RNA III is activated by at least two two-component systems, one encoded by the agr locus (AgrC-AgrA) and another encoded outside of this locus (TRAP-RAP). In this work, we developed new typing methods bas
American Society for Microbiology.