Competitive Pcr
Mostrando 1-12 de 276 artigos, teses e dissertações.
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1. Revealing potential long non-coding RNA biomarkers in lung adenocarcinoma using long non-coding RNA-mediated competitive endogenous RNA network
In our study, we aimed to reveal potential long non-coding RNAs (lncRNA) biomarkers in lung adenocarcinoma (LAD) using lncRNA-mediated competing endogenous RNAs (ceRNAs) network (LMCN). Competing lncRNA-mRNA interactions were identified using the hypergeometric test. Co-expression analysis for the competing lncRNA-mRNA interactions was implemented, and relyi
Braz J Med Biol Res. Publicado em: 07/08/2017
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2. Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics
BACKGROUND Real-time reverse transcription polymerase chain reaction (RT-PCR) is routinely used to detect viral infections. In Brazil, it is mandatory the use of nucleic acid tests to detect hepatitis C virus (HCV), hepatitis B virus and human immunodeficiency virus in blood banks because of the immunological window. The use of an internal control (IC) is
Mem. Inst. Oswaldo Cruz. Publicado em: 06/04/2017
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3. Molecular characterization of Torque teno virus and SEN virus co-infection with HIV in patients from Southern Iran
Introduction Torque teno virus (TTV) and SEN virus are circular single-stranded DNA viruses that cause blood-borne infections. The SEN virus (SEN-V) was originally detected in the serum of an injection drug user infected with human immunodeficiency virus (HIV). Recently TTV was discovered as a potential causative agent of non-A-E hepatitis. The aim of this
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-06
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4. Desenvolvimento de uma vacina recombinante para circovirose suína e ensaios para diagnóstico molecular de PCV2 / Development of a recombinant vaccine for porcine circovirus associated disease and molecular assays to detect PCV2
O circovírus suíno tipo 2 (PCV2) é o principal agente da síndrome multissistêmica do definhamento do suíno (SMDS), uma doença mundialmente disseminada e que provoca perdas econômicas significativas para a suinocultura. Visando contribuir no diagnóstico da síndrome, o presente trabalho padronizou e comparou testes para a detecção do PCV2. Para iss
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 2011
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5. Habitat partition among cervids in the Iguaçu National Park / Partição de habitats entre os cervídeos do Parque Nacional do Iguaçu
This study evaluated the determinant patterns of spatial distribution among the sympatric species Mazama americana and Mazama nana in the Iguaçu National Park under the principles of competitive exclusion and coexistence of ecologically related species. Three potential models of habitat partition were proposed to evaluate the relations among the occurrence
Publicado em: 2009
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6. Effect of growth hormone on fatty acid synthase gene expression in porcine adipose tissue cultures
We describe an efficient in vitro assay to test growth hormone effects on mRNA levels and fatty acid synthase (FAS, EC. 2.3.1.85) activity. Swine adipose tissue explants were long-term cultured in medium containing growth hormone and FAS mRNA levels and enzyme activity were measured. We quantified FAS transcripts by competitive reverse transcriptase PCR (RT-
Genetics and Molecular Biology. Publicado em: 2006
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7. Análise da variabilidade genética e Avaliação da fixação biológica de nitrogênio entre acessos de amendoim (Arachis hypogaea L.) / Analisys of genetic variability and assessment of biological nitrogen fixation among peanut (Arachis hypogaea L.) accessions
The peanut is a crop worldwide cultivated and it presents a high potential to biological nitrogen fixation (BNF). However peanut is considered a very promiscuous crop, what makes necessary a hard work of selection of rhizobial efficient and competitive strains to produce inoculants for this crop. The selection of an efficient association depends of the knowl
Publicado em: 2006
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8. Mecanismos reguladores da sintese de globinas : avaliação funcional da região R/PYR e analise da expressão genica diferencial na persistencia hereditaria de hemoglobina fetal e na delta-beta talassemia / Regulatory mechanisms of globin syntheis : functional evaluation of R/PYR region and differential gene expression analysis in hereditary persistence of etal hemoglobin and delta-beta thalassemia
The genetic mechanisms underlying the continued expression of the ?-globin genes during the adult stage in deletional hereditary persistence of fetal hemoglobin (HPFH) and ??-thalassemias are not completely understood. For deletional HPFH, three main hypotheses were proposed to explain the relationship between these deletions and the non-suppression of ? -ge
Publicado em: 2006
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9. Expression in normals and in subjects with schizophrenia of a novel gene fragment originally isolated from monozygotic twins discordant for schizophrenia
Differentially expressed clones from subtracted cDNA libraries of a pair of monozygotic twins discordant for schizophrenia have been reported in the literature. The clones were expressed in lymphocytes from the healthy twin, but not from the schizophrenic twin. In the current study, we assessed the expression of one of these clones, oksc12b, in 10 normal con
Genetics and Molecular Biology. Publicado em: 2004
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10. Standard-curve competitive RT-PCR quantification of myogenic regulatory factors in chicken embryos
The reverse transcription-polymerase chain reaction (RT-PCR) is the most sensitive method used to evaluate gene expression. Although many advances have been made since quantitative RT-PCR was first described, few reports deal with the mathematical bases of this technique. The aim of the present study was to develop and standardize a competitive PCR method us
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-12
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11. Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently in
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-02
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12. Development of a quantitative competitive reverse transcriptase polymerase chain reaction for the quantification of growth hormone gene expression in pigs
After the advent of the genome projects, followed by the discovery of DNA polymorphisms, basic understanding of gene expression is the next focus to explain the association between polymorphisms and the level of gene expression, as well as to demonstrate the interaction among genes. Among the various techniques for the investigation of transcriptional profil
Genetics and Molecular Biology. Publicado em: 2003