Complexing Dna
Mostrando 1-12 de 53 artigos, teses e dissertações.
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1. CARACTERIZAÇÃO DA CINÉTICA DE FORMAÇÃO E DA ESTABILIDADE DO COMPLEXO CROTAMINA-DNA VISANDO SUA UTILIZAÇÃO PARA O MELHORAMENTO DA TRANSGÊNESE ANIMAL / KINETIC CHARACTERIZATION OF TRAINING AND STABILITY OF COMPLEX crotamine-TARGETING YOUR DNA USE FOR THE IMPROVEMENT OF ANIMAL TRANSGENESIS
A crotamina é um polipeptídeo catiônico, originalmente isolado do veneno da cascavel da América do Sul (Crotalus durissus terrificus), constituído por 42 aminoácidos, rico em resíduos básicos e estabilizado por três pontes dissulfeto. Esta miotoxina possui a distinta habilidade de transpor membranas lipídicas e se acumular no núcleo de diversos ti
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 05/12/2011
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2. Membrane-bound fractions of R6K plasmid DNA in Escherichia coli.
The intracellular location of plasmid DNA has been of interest in an effort to understand the maintenance of these molecules. We have employed a simple procedure which enables us to isolate from exponentially grown cells on sucrose gradients membrane-complexed forms of R6K plasmid DNA. Electron micrographs identified the complexing of membrane fractions to c
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3. DNA orientation using specific avidin-ferritin biotin end labelling.
The orientation of DNA molecules has been determined by labelling one of the molecule end with a Biotin-labelled analog of dTTP (Bio-dUTP) and then by complexing the Bio-dUTP with Avidin-Ferritin. DNAs of phi X174, pBR322 and SV40 were end labelled with Bio-dUTP and imaged by Electron Microscopy (EM). This is a rapid, general method to unambiguously determin
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4. Rapid preparation of single stranded DNA from PCR products by streptavidin induced electrophoretic mobility shift.
Streptavidin induced electrophoretic mobility shift was used to prepare single stranded (ss) DNA amplified with the polymerase chain reaction in the presence of a biotinylated and a non-biotinylated primer. A variety of denaturing conditions, including incubation at 95 degrees C in 50% formamide can be used without disrupting the streptavidin-biotinylated-ss
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5. Early Stages in DNA Binding and Uptake During Genetic Transformation of Pneumococci
Ethylenediaminetetraacetate and other divalent-cation-complexing agents greatly stimulate the cellular binding of DNA molecules to competent pneumococci, while the appearance of genetic transformants and nuclease-resistant DNA binding are completely inhibited. Based on this finding, we developed an experimental system in which three early and consecutive sta
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6. Complementarity of RNA Produced by Enzymic Transcription of Native and Denatured B. subtilis DNA*
This paper describes the preparation and some of the properties of the RNA specimens synthesized with the aid of the RNA polymerase of E. coli by transcription of the following DNA templates: (a) undenatured B. subtilis DNA (yielding N-RNA); (b) separated strand fractions L and H isolated by chromatography of the denatured DNA on methylated albumin (yielding
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7. Resolution of the DNA strands of the specialized transducing bacteriophage lambda-h80C 1-857 dargF.
The DNA strands of lambdoid phages with deletions or substitutions of the guanine plus cytosine-rich region in the left arm are not resolvable by complexing with poly UG followed by centrifugation in CsCl. This work describes a completely general procedure for the strand resolution of these phages by hybridization with fragments of separated strands of the p
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8. A complex of single-strand binding protein and M13 DNA as hybridization probe.
Single-stranded DNA was complexed to the single-strand binding protein (SSB) of Escherichia coli in a mass ratio of 30:1. The protein moiety of this complex can be labelled by a number of methods of which we have chosen radio-iodination and biotinylation as examples. The SSB-M13 DNA complexes, labelled to high specific activities, were used as probes in hybr
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9. EVENTS OCCURRING DURING THE REPLICATION OF BACTERIOPHAGE T4 DNA*
Ten temperature-sensitive mutants of T4D have been examined to find the times in the lytic cycle at which a shift to a restrictive temperature no longer exerts a lethal effect. Nine of these mutants play a role in phage DNA synthesis. The results of these experiments suggest that the products of three of the genes tested—genes 42, 44, and 45—have control
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10. Metabolism of arginine-specific messenger ribonucleic acid in Escherichia coli K-12.
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of the level of messenger RNA (mRNA) transcribed from seven of the nine genes of the arginine regulon of Escherichia coli K-12. The quantity of RNA complexing with each of the separated DNA strands of the argA, argF, argE, and argCBH operons carried on specializ
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11. p53-catalyzed annealing of complementary single-stranded nucleic acids.
p53 has been reported to inhibit the DNA helicase intrinsic to simian virus 40 large tumor antigen (T antigen). We found that inhibition is not restricted to T antigen, but also affects several other DNA and RNA helicases. Complexing of the helicases by the p53 protein as a possible inactivation mechanism could be excluded. Instead, the anti-helicase activit
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12. p53 C-terminal interaction with DNA ends and gaps has opposing effect on specific DNA binding by the core
In addition to binding DNA in a sequence-specific manner, the p53 tumour suppressor protein can interact with damaged DNA. In order to understand which structural features in DNA the C-teminal domain recognises we have studied the interaction of p53 protein with different types of DNA oligonucleotides imitating damaged DNA. Here we show that one unpaired nuc
Oxford University Press.